Journal Article
| Title | Phox2b function in the enteric nervous system is conserved in zebrafish and is sox10-dependent. |
| Publication Type | Journal Article |
| Authors | Elworthy, S, Pinto, JP, Pettifer, A, M. Cancela, L, Kelsh, RN |
| Year of Publication | 2005 |
| Journal | Mech Dev |
| Volume | 122 |
| Issue | 5 |
| Date Published | 2005 May |
| Pagination | 659-69 |
| ISSN | 0925-4773 |
| Keywords | Amino Acid Sequence, Animals, Carrier Proteins, Central Nervous System, Chromosome Mapping, Enteric Nervous System, Gene Expression Regulation, Developmental, High Mobility Group Proteins, Homeodomain Proteins, In Situ Hybridization, Melanophores, Microscopy, Fluorescence, Models, Genetic, Molecular Sequence Data, Mutation, Neural Crest, Neurons, Phenotype, Phylogeny, Sequence Homology, Amino Acid, SOXE Transcription Factors, Stem Cells, Time Factors, Transcription Factors, Zebrafish, Zebrafish Proteins |
| Abstract | Zebrafish lacking functional sox10 have defects in non-ectomesenchymal neural crest derivatives including the enteric nervous system (ENS) and as such provide an animal model for human Waardenburg Syndrome IV. Here, we characterize zebrafish phox2b as a functionally conserved marker of the developing ENS. We show that morpholino-mediated knockdown of Phox2b generates fish modeling Hirschsprung disease. Using markers, including phox2b, we investigate the ontogeny of the sox10 ENS phenotype. As previously shown for melanophore development, ENS progenitor fate specification fails in these mutant fish. However, in addition, we trace back the sox10 mutant ENS defect to an even earlier time point, finding that most neural crest cells fail to migrate ventrally to the gut primordium. |
| DOI | 10.1016/j.mod.2004.12.008 |
| Sapientia | |
| Alternate Journal | Mech. Dev. |
| PubMed ID | 15817223 |
| Grant List | G0300415 / / Medical Research Council / United Kingdom |
