Expression analysis of two types of transcripts from circadian output gene lark in Bombyx mori. | - CCMAR -

Journal Article

TitleExpression analysis of two types of transcripts from circadian output gene lark in Bombyx mori.
Publication TypeJournal Article
AuthorsIwai, S, Takeda, M
Year of Publication2007
JournalComp Biochem Physiol B Biochem Mol Biol
Volume146
Issue4
Date Published2007 Apr
Pagination470-6
ISSN1096-4959
KeywordsAnimals, Blotting, Southern, Bombyx, Circadian Rhythm, Drosophila Proteins, Female, Gene Expression Regulation, Head, Insect Proteins, Larva, Male, Muscles, Ovary, RNA-Binding Proteins, Testis
Abstract

We analyzed expression patterns of lark (Bmlark) in Bombyx mori. Southern blot analysis demonstrated that Bmlark was a single copy gene. Northern blot analyses revealed two types of Bmlark transcripts, one being of 1.38 kb (Bmlark-PA) and the other of 0.85 kb (Bmlark-PB). Both transcripts were detected in the eggs, larval and adult heads, testes, ovaries and flight muscles. Both types of transcripts are constitutively expressed with no clear rhythmicity in the adult heads under light:dark (LD) cycles but the amount of the Bmlark-PA transcript was twice as much as that of the Bmlark-PB transcript in the adult heads throughout a day. Real-Time PCR assays also indicated constant expressions of the two types of Bmlark in the pupal brains under LD12:12 and LD16:8.

DOI10.1016/j.cbpb.2006.11.015
Sapientia

http://www.ncbi.nlm.nih.gov/pubmed/17287137?dopt=Abstract

Alternate JournalComp. Biochem. Physiol. B, Biochem. Mol. Biol.
PubMed ID17287137
CCMAR Authors