Vanadate proliferative and anti-mineralogenic effects are mediated by MAPK and PI-3K/Ras/Erk pathways in a fish chondrocyte cell line. | - CCMAR -

Journal Article

TitleVanadate proliferative and anti-mineralogenic effects are mediated by MAPK and PI-3K/Ras/Erk pathways in a fish chondrocyte cell line.
Publication TypeJournal Article
AuthorsTiago, DM, M. Cancela, L, Aureliano, M, Laizé, V
Year of Publication2008
JournalFEBS Lett
Volume582
Issue9
Date Published2008 Apr 16
Pagination1381-5
ISSN0014-5793
KeywordsAlkaline Phosphatase, Animals, Cell Line, Cell Proliferation, Chondrocytes, Fishes, MAP Kinase Signaling System, Phosphatidylinositol 3-Kinases, ras Proteins, Vanadates
Abstract

We recently reported proliferative and anti-mineralogenic effects of vanadate on fish chondrocytes and here we investigate the signalling pathways associated with these effects. Our data show that vanadate stimulates chondrocyte proliferation through the MAPK pathway, using signalling mechanisms similar to those used by IGF-1, while it inhibits chondrocyte differentiation/mineralization through a putative PI-3K/Ras/Erk signalling, a pathway shared with insulin. Our data also suggest that vanadate impairs ECM mineralization not only by interfering with regulatory pathways but also by inhibiting enzymatic activity of ALP. Finally, this work provides additional evidence for the conservation, throughout evolution, of mechanisms regulating chondrocyte proliferation and differentiation.

DOI10.1016/j.febslet.2008.03.025
Sapientia

http://www.ncbi.nlm.nih.gov/pubmed/18371308?dopt=Abstract

Alternate JournalFEBS Lett.
PubMed ID18371308
CCMAR Authors