Incubation temperature induces changes in muscle cellularity and gene expression in Senegalese sole (Solea senegalensis). | - CCMAR -

Journal Article

TitleIncubation temperature induces changes in muscle cellularity and gene expression in Senegalese sole (Solea senegalensis).
Publication TypeJournal Article
AuthorsCampos, C, Valente, LMP, Conceição, LEC, Engrola, S, Sousa, V, Rocha, E, Fernandes, JMO
Year of Publication2013
JournalGene
Volume516
Issue2
Date Published2013 Mar 10
Pagination209-17
ISSN1879-0038
KeywordsAnimals, Aquaculture, Cell Count, Embryo, Nonmammalian, Female, Flatfishes, Gene Expression Regulation, Developmental, Male, Muscle Development, Muscle Proteins, Muscles, MyoD Protein, Myogenic Regulatory Factor 5, Random Allocation, Temperature
Abstract

Fertilised eggs of Senegalese sole were incubated at 15, 18 or 21 °C, and after hatching all larvae were reared at 21 °C until 30 days post-hatch. By this point larvae from the 18 or 21 °C temperature groups had 11 and 9% more muscle fibres than those from 15 °C, respectively. Hyperplastic growth during metamorphosis was higher in larvae from 18 °C. Embryonic temperature induced gene expression changes, albeit with a variable pattern throughout development. Myf5, myod2, myHC and fst mRNA levels were significantly higher at several stages prior to hatching in embryos incubated at 21 °C, whereas hsp90AB and hsp70 transcripts were present at higher levels in the 15 °C group. Myf5, myod1, myod2, pax7, myog, fst, igf-II, igf1r, hsp90AA and hsp90AB were expressed at higher levels during early development, particularly during somitogenesis. In contrast, mrf4, myHC, mylc2, igf-I, mstn1 and hsp70 were up-regulated at later stages of larval development, namely during and after metamorphosis. This study is the first example of thermal plasticity of myogenesis with prolonged effect in a flatfish.

DOI10.1016/j.gene.2012.12.074
Sapientia

http://www.ncbi.nlm.nih.gov/pubmed/23291415?dopt=Abstract

Alternate JournalGene
PubMed ID23291415