Journal Article
Title | Experimental infection of European flat oyster Ostrea edulis with ostreid herpesvirus 1 microvar (OsHV-1μvar): Mortality, viral load and detection of viral transcripts by in situ hybridization. |
Publication Type | Journal Article |
Authors | Sanmartín, MLópez, Power, DM, de la Herrán, R, Navas, JI, Batista, FM |
Year of Publication | 2016 |
Journal | Virus Res |
Volume | 217 |
Date Published | 2016 Jun 2 |
Pagination | 55-62 |
ISSN | 1872-7492 |
Abstract | Ostreid herpesvirus 1 (OsHV-1) infections have been reported in several bivalve species. Mortality of Pacific oyster Crassostrea gigas spat has increased considerably in Europe since 2008 linked to the spread of a variant of OsHV-1 called μvar. In the present study we demonstrated that O. edulis juveniles can be infected by OsHV-1μvar when administered as an intramuscular injection. Mortality in the oysters injected with OsHV-1μvar was first detected 4 days after injection and reached 25% mortality at day 10. Moreover, the high viral load observed and the detection of viral transcripts by in situ hybridization in several tissues of dying oysters suggested that OsHV-1μvar was the cause of mortality in the O. edulis juveniles. This is therefore the first study to provide evidence about the pathogenicity of OsHV-1μvar in a species that does not belong to the Crassostrea genus. Additionally, we present a novel method to detect OsHV-1 transcripts in infected individuals' using in situ hybridization. |
DOI | 10.1016/j.virusres.2016.01.023 |
Sapientia | |
Alternate Journal | Virus Res. |
PubMed ID | 26945849 |