Local chromatin changes accompany the expression of the calbindin-D28K gene: tissue specificity and effect of vitamin D activation. | - CCMAR -

Journal Article

TitleLocal chromatin changes accompany the expression of the calbindin-D28K gene: tissue specificity and effect of vitamin D activation.
Publication TypeJournal Article
AuthorsM. Cancela, L, Ishida, H, Bishop, JE, Norman, AW
Year of Publication1992
JournalMol Endocrinol
Volume6
Issue3
Date Published1992 Mar
Pagination468-75
ISSN0888-8809
KeywordsAnimals, Calbindins, Calcitriol, Chickens, Chromatin, Deoxyribonuclease I, Gene Expression Regulation, Organ Specificity, Restriction Mapping, S100 Calcium Binding Protein G
Abstract

The high affinity calcium-binding protein calbindin-D28K is one of the known proteins transcriptionally up-regulated by the hormonally active form of vitamin D3, 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]. This regulation is tissue specific, since in the absence of 1,25-(OH)2D3, the expression of calbindin-D28K is virtually abolished in intestine, whereas it is decreased, but clearly detectable, in kidney, and it remains present at its highest level in cerebellum. Several studies have shown that there is a strong correlation between an increase in the sensitivity to nuclease digestion of a given gene locus and its potential for transcription. Furthermore, hypersensitive sites have often been mapped to regions of DNA including or surrounding sequences known to be important for the regulation of gene transcription. In this study we have scanned the 5'-end and flanking DNA of the calbindin-D28K gene for the presence of DNase-I-hypersensitive (DH) sites in order to localize possible regulatory regions involved in the tissue-specific and hormone-dependent regulation of this gene. We have found that in tissues where calbindin is not expressed, such as liver, no DH sites could be detected. In cerebellum, the same set of DH sites was observed in the presence or absence of 1,25-(OH)2D3 treatment, reflecting the vitamin D-independent expression of the calbindin gene in this tissue. A more complex pattern of DH sites was found in intestine, independently of the vitamin D status of the animal.(ABSTRACT TRUNCATED AT 250 WORDS)

DOI10.1210/mend.6.3.1584219
Sapientia

http://www.ncbi.nlm.nih.gov/pubmed/1584219?dopt=Abstract

Alternate JournalMol. Endocrinol.
PubMed ID1584219
Grant ListDK-09012-028 / DK / NIDDK NIH HHS / United States
CCMAR Authors