UNIVERSITY EDUCATION

2009-2013  B.Sc. Biological technology, Wenzhou University.

2014-2017  M.Sc. Aquaculture, Shanghai Ocean University.

2017-2020  P.h.D. Aquaculture, Shanghai Ocean University.

2018-2023  P.h.D. Biological Science, University do Algarve.

ACADEMIC EXPERIENCE

During 2010 to 2014 years I began to participate in academic research. I mainly worked on marine ecology of community structure of macrozoobenthos in intertidal zone. In addition, I participated in several items that reproductive biology research of gecko and ophidian.

During 2014 to 2018 years I mainly worked on razor clam (Sinonovacula constricta) in lab of professor Li (Shanghai Ocean University). There have four parts which are consisted in my work. Part one is my thesis which research in the innate immune of complement system (complement C3 and B factor) in razor clam. Part two, I achieved primary cells (hemolymph, liver and mantle) and tissues (heart, liver and mantle) culture of razor clam. Part three, I achieved artificial breeding of razor clam in lab, it can help us conveniently acquire experiment material. Part four, inland salinity water and freshwater were used to raise razor clam. The purpose of this part is to explore the possibility of inland of China farming razor clam.

During 2018 to 2023 years I mainly worked on mussel (Mytilus galloprovincalis) and oyster (Magallana gigas) in lab of professor Deborah M Power (University do Algarve, The Centre of Marine Sciences). There have four parts which are consisted in my thesis. Part one, discuss the content of chitin in bivalve with different shell materials, the evolution of chitin metabolism-related enzymes (chitinase and chitin synthase) in bivalve, and further explore whether ocean acidification (OA) has general impact on bivalve shell organic matter-associated enzymes (chitin turnover). Part two, further explore the toolbox genes related to the formation of asymmetric valve in bivalve, as well as explore the molecular evolution of asymmetric valve related genes and describe the molecular regulatory pathways. Part three, to explore the impact of global climate change (OA) on different species of bivalve, and to assess the potential impact of the model of "bivalve physiology-shell-ocean carbon cycle-human". Part four, the diverse and size-dependent impact of PS-NPs on fibroblast cell lines from fish and human, revealing differences in proliferation, migration and enzyme activity. These findings stress the need for further exploration of NP interactions with different cell types to better understand potential implication for human health.

SKILLS

PCR, QRT-PCR, construction of the recombinant plasmid, WB (western blot), IP (immunoprecipitation), co-IP (co-immunoprecipitation), protein expression, protein purification, overexpression, gene silencing by siRNA, laser confocal, phagocytosis, chemotaxis, hemolysis, molecular evolution research, RNA sequencing (RNAseq), Assay for Transposase-Accessible Chromatin using sequencing (ATACseq), single-cell RNA sequencing (scRNA-seq), cell culture, enzyme activity, artificial breeding juvenile of razor clam in lab and breeding algae.

PUBLICATIONS

1. Sousa C S V, Peng M, Guerreiro PM, et al. Differential tissue immune stimulation through immersion in bacterial and viral agonists in the Antarctic Notothenia rossii. Fish & Shellfish Immunology, 2024: 109516.
2. Maoxiao Peng, Félix R C, Canário A V M, et al. The physiological effect of polystyrene nanoplastic particles on fish and human fibroblasts. Science of The Total Environment, 2024: 169979.
3. Maoxiao Peng, J.C.R Cardoso, G Pearson, et al. Core genes of biomineralization and cis-regulatory long non-coding RNA regulate shell growth in bivalves. Journal of Advanced Research, 2023.
4. Maoxiao Peng, Zhi Li, Cardoso J.C.R, et al. Domain-Dependent Evolution Explains Functional Homology of Protostome and Deuterostome Complement C3-Like Proteins. (2022) Frontiers in immunology, 13: 840861-840861.
5. Maoxiao Peng, Zhengmin Liu, Zhi Li, et al. The temptin gene of the clade Lophotrochozoa is involved in formation of the prismatic layer during biomineralization in molluscs. (2021) International Journal of Biological Macromolecules, 188: 800-810.
6. Zhi Li, Maoxiao Peng, Power DM, et al. RNAi-mediated knock-down of the dopamine beta-hydroxylase gene changes growth of razor clams. (2021) Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 252: 110534. (co-first author)
7. Zhi Li, João CR Cardoso, Maoxiao Peng et al. Evolution and potential function in molluscs of neuropeptide and receptor homologues of the insect allatostatins. (2021). Frontiers in endocrinology 1120.
8. Zhi Li, Donghong Niu, Maoxiao Peng, Ya Xiong, Jie Ji, Zhiguo Dong, Jiale Li. (2020). Dopamine beta-hydroxylase and its role in regulating the growth and larval metamorphosis in Sinonovacula constricta. Gene. 737: 144418.
9. Tianyi Lan, Zhi Li, Maoxiao Peng, Donghong Niu, Yan Li, Jiale Li. (2020). A four-CRD C-type lectin from razor clam Sinonovacula constricta mediates agglutination and phagocytosis. Gene. 728: 144287.
10. Dang Hai Nguyen, Donghong Niu, Zhiyi Chen, Maoxiao Peng, Nga Thi Tran, Jiale Li. Classification and morphology of circulating haemocytes in the razor clam Sinonovacula constricta. (2020). Aquaculture and Fisheries. DOI: https://doi.org/10.1016/j.aaf.2020.06.007.
11. Zhi Li, Maoxiao Peng, Deborah Mary Power, Donghong Niu *, Zhiguo Dong, Jiale Li*. (2020). RNAi-mediated knock-down of the dopamine beta-hydroxylase gene changes growth of razor clams. 252: 110534. (co-first author)
12. Peng Maoxiao, Li Zhi, Niu Donghong, Liu Xiaojun, Dong Zhiguo, Li Jiale*. (2020) Inland alkaline brackish water aquaculture of juvenile razor clam Survival, growth, physiology and immune responses. Aquaculture reports. 18: 100463. https://doi.org/10.1016/j.aqrep.2020.100463
13. João CR Cardoso*, Rute C Félix, Vinícius Ferreira, Maoxiao Peng, Xushuai Zhang, Deborah M Power*. (2020). The calcitonin-like system is an ancient regulatory system of biomineralization. Scientific reports. DOI: https://doi.org/10.1038/s41598-020-64118-w
14. Peng maoxiao, Zhi Li, Xiaojun Liu, Tianyi Lan, Donghong Niu, Bo Ye, Zhiguo Dong, Li Jiale*. (2020). Survival, growth, and physiology of the juvenile razor clam (Sinonovacula constricta) under Na+/K+ ratio stress. Aquaculture Research DOI: 10.1111/are.14429.
15. Li Zhi, Peng Maoxiao, Ye Bo, Niu Donghong, Lan Tianyi, Liu Xiaojun and Li Jiale. (2020) Acute of low salinity on survival, Na + /K + -ATPase activity and hemolymph phagocytic ability in the razor clam, Sinonovacula constricta. (in Chinese)
16. Peng maoxiao, Zhi Li, Donghong Niu, Xiaojun Liu, Zhiguo Dong, Li Jiale*. (2019). Complement factor B/C2 in molluscs regulates agglutination and illuminates evolution of the Bf/C2 family. The FASEB Journal. 33. doi: 10.1096/fj.201901142RR.
17. Peng maoxiao, Li Zhi, Liu Xiaojun, Niu Donghong, Lan Tianyi, Ye Bo, Dong Zhiguo Li Jiale. (2019). Tolerance, growth, and physiological responses of the juvenile razor clam (Sinonovacula constricta) to environmental Ca2+ and Mg2+ concentrations. Frontiers in Physiology. 10:911. doi: 10.3389/fphys.2019.00911.
18. Peng, Maoxiao, Liu, Xiaojun, Niu, Donghong, Ye, Bo, Lan, Tianyi, Dong, Zhiguo, Li, Jiale. (2019). Survival, growth and physiology of marine bivalve (Sinonovacula constricta) in long-term low-salt culture. Scientific Reports. 9:2819. doi: 10.1038/s41598-019-39205-2
19. Ye bo, Cheng zhiyang, Peng maoxiao, Niu donghong, Liu xiaojun, Lan tianyi. (2019) Effects of pH and carbonate alkalinity on survival rate, Na+/K+-ATPase activity and phagocytic ability of the razor clam (Sinonovacula constricta). Journal of Fisheries of China. 43: 1723-1732. (In Chinese)
20. Peng maoxiao, Ye bo, Liu xiaojun, Niu donghong, Lan tianyi, Dong zhiguo, Li jiale.

(2018). Effects of alkalinity and pH on survival, growth, and enzyme activities in juveniles of the razor clam, Sinonovacula constricta. Frontiers in physiology. 9: 552. Doi: 10.3389/fphys.2018.00552

21. Niu donghong, Xiong ya, Peng maoxiao, Chen zhiyi, Lan tianyi, Li jiale. (2018). Hemolytic reactions in the hemolymph of bivalve Sinonovacula constricta shows a complement-like activity. Fish & Shellfish Immunology. 79: 11-17.
22. Peng maoxiao, Niu donghong, Chen zhiyi, Lan tianyi, Dong zhiguo, Tran Thi-Nga, Li jiale. (2017) Expression of a novel complement C3 gene in the razor clam Sinonovacula constricta and its role in innate immune response and hemolysis. Developmental and Comparative Immunology. 73: 184-192.
23. Peng maoxiao, Niu donghong, Wang fei, Chen zhiyi, Li jiale. (2016) Complement C3 gene: Expression characterization and innate immune response in razor clam Sinonovacula constricta. Fish & Shellfish Immunology. 55: 223-232.
24. Peng maoxiao, Niu donghong, Li jiale. (2016) Complement system in marine invertebrate. Marine Fisheries. 38:320-328. (In Chinese)
25. Peng maoxiao, Qian peili, Zhang yongpu, Wang tiegan, Zhou huabin. (2013) Community structure of macrozoobenthos in rocky intertidal zone of uninhabited islands in Dongtou County, Zhejiang Province of East China in summer. Chinese Journal of Ecology. 32(9): 2469-2479. (In Chinese)

Patent

patents for utility models

1. Li jiale, Peng maoxiao, Niu donghong, Xie shumei, Wang ze, Wang fei, Wei kanyun (2015). A fast body measuring instrument for mollusk.
2. Li jiale, Peng maoxiao, Niu donghong, Xie shumei, Wang fei, Wang ze, Du yunchao, Huang wenji (2015). A fast filling instrument for Pipette Tips.
3. Li jiale, Peng maoxiao, Niu donghong, Du yunchao, Xie shumei (2016). A hemolymph extracting instrument for bivalve.

patents for invention

1. Li jiale, Peng maoxiao, Niu donghong, Xie shumei, Huang wenji, Wang fei, Wang ze, Wei kanyun, Du yunchao (2015). Method and instrument for agarose gel imaging and auto-incision.
2. Niu donghong, Du yunchao, Peng maoxiao, Shang lixin, Li jiale, Wei kanyun (2016). A hemolymph extracting method for bivalve.
3. Li jiale, Peng maoxiao, Liu xiaojun, Niu donghong, Ye bo, Lan tianyi, Li yan, Bai yuqi, Li zhi, Xiong ya (2018). A low-salt acclimation method for Sinonovacula constricta seedlings. (Under review)

Project

Title: Development of technological tools to improve and reduce health risks for bivalve consumption in angola.

Date: 01/2022 to 12/2024.

Members: Joao Carlos dos Reis Cardoso (holder), Rute Castelo Félix, Maoxiao Peng, Adelino Vicente Mendonça Canario, Deborah Mary Power

Funding: FCT - Fundação para a Ciência e Tecnologia