Chromatography and mass spectrometry are very powerful techniques. Their use in Marine Sciences is currently under expansion. The Analytical and Structural Chemistry Platform of EMBRC.PT has developed several protocols for the identification and quantification of bioactive molecules in marine matrices. These Knowledge Exchange and Training activities will give an overview of liquid chromatography and mass spectrometry methods to researchers and demonstrate their potential in Marine Sciences by running specific examples. Early-career researchers and prospective users are particularly welcomed. Participants are encouraged to send selected samples for analysis during the course.