Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis. Functional analysis of the promoter identifies a calcium sensitive region required for basal activity.

Conceição, Natércia; Henriques, Nuno M; Ohresser, Marc C P; Hublitz, Philip; Schüle, Roland; M. Leonor Cancela; M. Leonor Cancela; M. Leonor Cancela; Nitschke, Yvonne; Pasch, Andreas; Quaglino, Daniela; Rutsch, Frank; Shanahan, Catherine; Sorribas, Victor; Szeri, Flora; Valdivielso, Pedro; Vanakker, Olivier; Kempf, Hervé

Natércia Conceição

Posição

Investigador

Grau Académico

Doutoramento

Líder do Grupo

Comparative, Adaptive and Functional Skeletal Biology

Grupo

Comparative, Adaptive and Functional Skeletal Biology

Categoria

Assistant

Instituição

Universidade do Algarve

Localização

Faro, Gambelas Campus, UAlg

Gabinete

2.76

Contacto

+351 289800057

Extensão

7057

nconcei@ualg.pt

Keywords

zebrafish; extracellular matrix mineralization; cell culture; gene regulation; fish models of human diseases

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ORCID ID

Scopus

Organiza

Projetos

Coordenação de Projetos

	Título	Coordenador	Início	Fim	Financiamento	Orçamento	Orçamento Total
IBIGEN-LHON	Investigação bigenómica translacional na Neuropatia Ótica Hereditária de Leber: Correlação Genótipo-Fenótipo	Natércia Conceição	04/2013	08/2015	FCT - Fundação para a Ciência e Tecnologia	7k€
GLARE	Padrão de expressão espaço-temporal da Matrix Gla Protein (MGP) e sua regulação durante o desenvolvimento em X. laevis.	Natércia Conceição	06/2005	06/2008	FCT - Fundação para a Ciência e Tecnologia	72k€

Projects Membership

	Título	Coordenador	Início	Fim	Orçamento	Orçamento Total
SaMGP	Effect of extracellular calcium on MGP gene expression	Vincent Laizé	03/2003	03/2006	100k€	100k€

Eventos

Tipo de Evento

Outros Eventos

Apresentação da tese de mestrado de Michele Loiodice

Esta sexta-feira, o aluno de mestrado Michele Loiodice, irá apresentar e defender a sua tese na Universidade do Algarve às 14:00h.

Grupo

Comparative, Adaptive and Functional Skeletal Biology

Sex, 08/11/2019

Notícias

News

Especialistas discutem esqueleto e problemas ósseos esta semana

O CCMAR organiza esta semana a 5ª edição da Conferência “Interdisciplinary Approaches in Fish Skeletal Biology (IAFSB)” em Tavira, Portugal.

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Publicações

Year of Publication
2023	Varela D, Varela T, Conceição N, M. Cancela L. Regulation of human ZNF687, a gene associated with Paget's disease of bone. The International Journal of Biochemistry & Cell Biology. 2023;154:106332. doi:10.1016/j.biocel.2022.106332 Ler mais acerca de Regulation of human ZNF687, a gene associated with Paget's disease of bone Google Scholar DOI
2023	Santos JMA, Laizé V, Gavaia PJ, Conceição N, M. Cancela L. Zebrafish Models to Study Ectopic Calcification and Calcium-Associated Pathologies. International Journal of Molecular Sciences. 2023;24(4):3366. doi:10.3390/ijms24043366 Ler mais acerca de Zebrafish Models to Study Ectopic Calcification and Calcium-Associated Pathologies Google Scholar DOI
2023	Adrião A, Mariano S, Mariano J, et al. mef2ca and mef2cb Double Mutant Zebrafish Show Altered Craniofacial Phenotype and Motor Behaviour. Biomolecules. 2023;13(5):805. doi:10.3390/biom13050805 Ler mais acerca de mef2ca and mef2cb Double Mutant Zebrafish Show Altered Craniofacial Phenotype and Motor Behaviour Google Scholar DOI
2022	Adrião A, Santana I, Ribeiro C, M. Cancela L, Conceição N, Grazina M. Identification of a novel mutation in MEF2C gene in an atypical patient with frontotemporal lobar degeneration. Neurological Sciences. 2022;43(1):319 - 326. doi:10.1007/s10072-021-05269-0 Ler mais acerca de Identification of a novel mutation in MEF2C gene in an atypical patient with frontotemporal lobar degeneration Google Scholar DOI
2022	Silva IAL, Varela D, M. Cancela L, Conceição N. Zebrafish optineurin: genomic organization and transcription regulation. Genome. 2022;65(10):513 - 523. doi:10.1139/gen-2022-0019 Ler mais acerca de Zebrafish optineurin: genomic organization and transcription regulation Google Scholar DOI
2022	Simão M, Conceição N, Imaginário S, Amaro J, Cancela MLeonor. Lab-it is taking molecular genetics to school. BioChem. 2022;2(2):160 - 170. doi:10.3390/biochem2020011 Ler mais acerca de Lab-it is taking molecular genetics to school Google Scholar DOI
2022	Varela T, Varela D, Martins G, Conceição N, M. Cancela L. Cdkl5 mutant zebrafish shows skeletal and neuronal alterations mimicking human CDKL5 deficiency disorder. Scientific Reports. 2022;12(1). doi:10.1038/s41598-022-13364-1 Ler mais acerca de Cdkl5 mutant zebrafish shows skeletal and neuronal alterations mimicking human CDKL5 deficiency disorder Google Scholar DOI
2021	Cancela ML, Laizé V, Conceição N, Kempf H, Murshed M. Keutel syndrome, a review of 50 years of literature. Frontiers in Cell and Developmental Biology. 2021;9:642136. doi:10.3389/fcell.2021.642136 Ler mais acerca de Keutel syndrome, a review of 50 years of literature Google Scholar DOI
2021	Varela T, Conceição N, Laizé V, Cancela ML. Transcriptional regulation of human DUSP4 gene by cancer‐related transcription factors. Journal of Cellular Biochemistry. 2021;122(10):1556 - 1566. doi:10.1002/jcb.v122.1010.1002/jcb.30078 Ler mais acerca de Transcriptional regulation of human DUSP4 gene by cancer‐related transcription factors Google Scholar DOI
2021	Varela D, Varela T, Conceição N, et al. Functional analysis of two novel TBX5 variants present in individuals with Holt–Oram syndrome with different clinical manifestations. Molecular Genetics and Genomics. 2021;296(4):809 - 821. doi:10.1007/s00438-021-01781-2 Ler mais acerca de Functional analysis of two novel TBX5 variants present in individuals with Holt–Oram syndrome with different clinical manifestations Google Scholar DOI
2021	Varela D, Conceição N, M. Cancela L. Transcriptional regulation of human T-box 5 gene (TBX5) by bone- and cardiac-related transcription factors. Gene. 2021;768:145322. doi:10.1016/j.gene.2020.145322 Ler mais acerca de Transcriptional regulation of human T-box 5 gene (TBX5) by bone- and cardiac-related transcription factors Google Scholar DOI
2020	Caiado H, Conceição N, Tiago D, et al. Evaluation of MGP gene expression in colorectal cancer. Gene. 2020;723:144120. doi:10.1016/j.gene.2019.144120 Ler mais acerca de Evaluation of MGP gene expression in colorectal cancer Google Scholar DOI
2020	Caiado H, Conceição N, Tiago D, et al. Data on the evaluation of FGF2 gene expression in Colorectal Cancer. Data in Brief. 2020;31:105765. doi:10.1016/j.dib.2020.105765 Ler mais acerca de Data on the evaluation of FGF2 gene expression in Colorectal Cancer Google Scholar DOI
2020	Varela T, Laizé V, Conceição N, et al. Expression of DUSP4 transcript variants as a potential biomarker for colorectal cancer. Biomarkers in Medicine. 2020;14(8):639 - 650. doi:10.2217/bmm-2019-0369 Ler mais acerca de Expression of DUSP4 transcript variants as a potential biomarker for colorectal cancer Google Scholar DOI
2019	Bäck M, Aranyi T, M. Cancela L, et al. Endogenous Calcification Inhibitors in the Prevention of Vascular Calcification: A Consensus Statement From the COST Action EuroSoftCalcNet. Frontiers in Cardiovascular Medicine. 2019;5. doi:10.3389/fcvm.2018.00196 Ler mais acerca de Endogenous Calcification Inhibitors in the Prevention of Vascular Calcification: A Consensus Statement From the COST Action EuroSoftCalcNet Google Scholar DOI
2018	Fazenda C, Martins G, Gavaia P, M. Cancela L, Conceição N. Generation of zebrafish Danio rerio (Hamilton, 1822) transgenic lines overexpressing a heat-shock mediated Gla-rich protein. Journal of Applied Ichthyology. 2018. doi:10.1111/jai.13662 Ler mais acerca de Generation of zebrafish Danio rerio (Hamilton, 1822) transgenic lines overexpressing a heat-shock mediated Gla-rich protein Google Scholar DOI
2018	Silva IAL, Conceição N, Gagnon E, Brown JP, M. Cancela L, Michou L. Molecular effect of an OPTN common variant associated to Paget's disease of bone. PLOS ONE. 2018;13(5):e0197543. doi:10.1371/journal.pone.0197543.s007 Ler mais acerca de Molecular effect of an OPTN common variant associated to Paget's disease of bone Google Scholar DOI
2018	Vitorino M, Cunha N, Conceição N, M. Cancela L. Expression pattern of cdkl5 during zebrafish early development: implications for use as model for atypical Rett syndrome. Molecular Biology Reports. 2018. doi:10.1007/s11033-018-4180-1 Ler mais acerca de Expression pattern of cdkl5 during zebrafish early development: implications for use as model for atypical Rett syndrome Google Scholar DOI
2018	Vitorino M, Conceição N, M. Cancela L. Zebrafish (Danio rerio, Hamilton-Buchanan, 1822) as a model to study bone diseases associated with Rett syndrome. Journal of Applied Ichthyology. 2018;34(2):489 - 500. doi:10.1111/jai.13667 Ler mais acerca de Zebrafish (Danio rerio, Hamilton-Buchanan, 1822) as a model to study bone diseases associated with Rett syndrome Google Scholar DOI
2018	Silva IAL, Conceição N, Gagnon E, et al. Effect of genetic variants of OPTN in the pathophysiology of Paget's disease of bone. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 2018;1864(1):143 - 151. doi:10.1016/j.bbadis.2017.10.008 Ler mais acerca de Effect of genetic variants of OPTN in the pathophysiology of Paget's disease of bone Google Scholar DOI
2016	Adrião A, Conceição N, M. Cancela L. MEF2C orthologues from zebrafish: Evolution, expression and promoter regulation. Arch Biochem Biophys. 2016;591:43-56. doi:10.1016/j.abb.2015.12.004 Ler mais acerca de MEF2C orthologues from zebrafish: Evolution, expression and promoter regulation. Google Scholar PubMed DOI
2016	Correia E, Conceição N, M. Cancela L, Belo JA. Matrix Gla Protein expression pattern in the early avian embryo. Int J Dev Biol. 2016;60(1-3):71-6. doi:10.1387/ijdb.150365jb Ler mais acerca de Matrix Gla Protein expression pattern in the early avian embryo. Google Scholar PubMed DOI
2016	Tiago DM, Conceição N, Caiado H, Laizé V, M. Cancela L. Matrix Gla protein repression by miR-155 promotes oncogenic signals in breast cancer MCF-7 cells. FEBS Letters. 2016;590(8):1234-1241. doi:10.1002/1873-3468.12155 Ler mais acerca de Matrix Gla protein repression by miR-155 promotes oncogenic signals in breast cancer MCF-7 cells Google Scholar PubMed DOI
2015	Fazenda C, Conceição N, M. Cancela L. Transcription factors from Sox family regulate expression of zebrafish Gla-rich protein 2 gene. Gene. 2015;572(1):57-62. doi:10.1016/j.gene.2015.06.079 Ler mais acerca de Transcription factors from Sox family regulate expression of zebrafish Gla-rich protein 2 gene. Google Scholar PubMed DOI
2015	Silva IAL, Conceição N. Cloning, characterization and analysis of the 5′ regulatory region of zebrafish xpd gene. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 2015;185:47 - 53. doi:10.1016/j.cbpb.2015.04.003 Ler mais acerca de Cloning, characterization and analysis of the 5′ regulatory region of zebrafish xpd gene Google Scholar DOI
2015	Simões B, Conceição N, Matias AC, Bragança J, Kelsh RN, M. Cancela L. Molecular characterization of cbfβ gene and identification of new transcription variants: implications for function. Arch Biochem Biophys. 2015;567:1-12. doi:10.1016/j.abb.2014.12.023 Ler mais acerca de Molecular characterization of cbfβ gene and identification of new transcription variants: implications for function. Google Scholar PubMed DOI
2014	M. Cancela L, Laizé V, Conceição N. Matrix Gla protein and osteocalcin: from gene duplication to neofunctionalization. Arch Biochem Biophys. 2014;561:56-63. doi:10.1016/j.abb.2014.07.020 Ler mais acerca de Matrix Gla protein and osteocalcin: from gene duplication to neofunctionalization. Google Scholar PubMed DOI
2014	Simões B, Conceição N, Kelsh RN, M. Cancela L. Identification of cis -regulatory elements in the upstream regions of zebrafish runx3 through in silico analysis: implications for function. Journal of Applied Ichthyology. 2014;30(4):661 - 670. doi:10.1111/jai.2014.30.issue-410.1111/jai.12536 Ler mais acerca de Identification of cis -regulatory elements in the upstream regions of zebrafish runx3 through in silico analysis: implications for function Google Scholar DOI
2014	Silva IAL, Conceição N, Michou L, M. Cancela L. Can zebrafish be a valid model to study Paget's disease of bone?. Journal of Applied Ichthyology. 2014;30(4):678 - 688. doi:10.1111/jai.2014.30.issue-410.1111/jai.12523 Ler mais acerca de Can zebrafish be a valid model to study Paget's disease of bone? Google Scholar DOI
2014	Silva IAL, Cox CJ, Leite RB, M. Cancela L, Conceição N. Evolutionary conservation of TFIIH subunits: implications for the use of zebrafish as a model to study TFIIH function and regulation. Comp Biochem Physiol B Biochem Mol Biol. 2014;172-173:9-20. doi:10.1016/j.cbpb.2014.03.007 Ler mais acerca de Evolutionary conservation of TFIIH subunits: implications for the use of zebrafish as a model to study TFIIH function and regulation. Google Scholar PubMed DOI
2013	Conceição N, Viegas M, Fidalgo J, M. Cancela L. Development and characterization of Xl1, a Xenopus laevis chondrocyte-like cell culture. Mol Cell Biochem. 2013;373(1-2):41-51. doi:10.1007/s11010-012-1473-x Ler mais acerca de Development and characterization of Xl1, a Xenopus laevis chondrocyte-like cell culture. Google Scholar PubMed DOI
2012	Conceição N, Fazenda C, M. Cancela L. Comparative gene promoter analysis: an in silico strategy to identify candidate regulatory factors for Gla Rich Protein. Journal of Applied Ichthyology. 2012;28(3):372 - 376. doi:10.1111/j.1439-0426.2012.02001.x Ler mais acerca de Comparative gene promoter analysis: an in silico strategy to identify candidate regulatory factors for Gla Rich Protein Google Scholar DOI
2012	Fazenda C, Silva IAL, M. Cancela L, Conceição N. Molecular characterization of two paralog genes encoding Gla-rich protein (Grp) in zebrafish. Journal of Applied Ichthyology. 2012;28(3):377 - 381. doi:10.1111/j.1439-0426.2012.02004.x Ler mais acerca de Molecular characterization of two paralog genes encoding Gla-rich protein (Grp) in zebrafish Google Scholar DOI
2012	Michou L, Conceição N, Morissette J, et al. Genetic association study of UCMA/GRP and OPTN genes (PDB6 locus) with Paget's disease of bone. Bone. 2012;51(4):720-8. doi:10.1016/j.bone.2012.06.028 Ler mais acerca de Genetic association study of UCMA/GRP and OPTN genes (PDB6 locus) with Paget's disease of bone. Google Scholar PubMed DOI
2012	M. Cancela L, Conceição N, Laizé V. Gla-rich protein, a new player in tissue calcification?. Adv Nutr. 2012;3(2):174-81. doi:10.3945/an.111.001685 Ler mais acerca de Gla-rich protein, a new player in tissue calcification? Google Scholar PubMed DOI
2012	Silva IAL, M. Cancela L, Conceição N. Molecular cloning and expression analysis of xpd from zebrafish (Danio rerio). Mol Biol Rep. 2012;39(5):5339-48. doi:10.1007/s11033-011-1333-x Ler mais acerca de Molecular cloning and expression analysis of xpd from zebrafish (Danio rerio). Google Scholar PubMed DOI
2010	Conceição N, Cox CJ, Simões B, Viegas M, M. Cancela L. Comparative promoter analysis and its application to the identification of candidate regulatory factors of cartilage-expressed genes. Journal of Applied Ichthyology. 2010;26(2):245 - 250. doi:10.1111/jai.2010.26.issue-210.1111/j.1439-0426.2010.01415.x Ler mais acerca de Comparative promoter analysis and its application to the identification of candidate regulatory factors of cartilage-expressed genes Google Scholar DOI
2010	Fazenda C, Simões B, Kelsh RN, M. Cancela L, Conceição N. Dual transcriptional regulation by runx2 of matrix Gla protein in Xenopus laevis. Gene. 2010;450(1-2):94-102. doi:10.1016/j.gene.2009.10.007 Ler mais acerca de Dual transcriptional regulation by runx2 of matrix Gla protein in Xenopus laevis. Google Scholar PubMed DOI
2008	Conceição N, Laizé V, Simões B, Pombinho AR, M. Cancela L. Retinoic acid is a negative regulator of matrix Gla protein gene expression in teleost fish Sparus aurata. Biochim Biophys Acta. 2008;1779(1):28-39. doi:10.1016/j.bbagrm.2007.11.003 Ler mais acerca de Retinoic acid is a negative regulator of matrix Gla protein gene expression in teleost fish Sparus aurata. Google Scholar PubMed DOI
2006	Simões B, Conceição N, Viegas CSB, et al. Identification of a promoter element within the zebrafish colXalpha1 gene responsive to runx2 isoforms Osf2/Cbfa1 and til-1 but not to pebp2alphaA2. Calcif Tissue Int. 2006;79(4):230-44. doi:10.1007/s00223-006-0111-6 Ler mais acerca de Identification of a promoter element within the zebrafish colXalpha1 gene responsive to runx2 isoforms Osf2/Cbfa1 and til-1 but not to pebp2alphaA2. Google Scholar PubMed DOI
2005	Pinto JP, Conceição NM, Viegas CSB, et al. Identification of a new pebp2alphaA2 isoform from zebrafish runx2 capable of inducing osteocalcin gene expression in vitro. J Bone Miner Res. 2005;20(8):1440-53. doi:10.1359/JBMR.050318 Ler mais acerca de Identification of a new pebp2alphaA2 isoform from zebrafish runx2 capable of inducing osteocalcin gene expression in vitro. Google Scholar PubMed DOI
2005	Conceição N, Silva AC, Fidalgo J, Belo JA, M. Cancela L. Identification of alternative promoter usage for the matrix Gla protein gene. Evidence for differential expression during early development in Xenopus laevis. FEBS J. 2005;272(6):1501-10. doi:10.1111/j.1742-4658.2005.04590.x Ler mais acerca de Identification of alternative promoter usage for the matrix Gla protein gene. Evidence for differential expression during early development in Xenopus laevis. Google Scholar PubMed DOI
2003	Pinto JP, Conceição N, Gavaia P, M. Cancela L. Matrix Gla protein gene expression and protein accumulation colocalize with cartilage distribution during development of the teleost fish Sparus aurata. Bone. 2003;32(3):201-10. Ler mais acerca de Matrix Gla protein gene expression and protein accumulation colocalize with cartilage distribution during development of the teleost fish Sparus aurata. Google Scholar PubMed
2002	Conceição N, Henriques NM, Ohresser MCP, Hublitz P, Schüle R, M. Cancela L. Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis. Functional analysis of the promoter identifies a calcium sensitive region required for basal activity. Eur J Biochem. 2002;269(7):1947-56. Ler mais acerca de Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis. Functional analysis of the promoter identifies a calcium sensitive region required for basal activity. Google Scholar PubMed

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