Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis. Functional analysis of the promoter identifies a calcium sensitive region required for basal activity.

Conceição, Natércia; Henriques, Nuno M; Ohresser, Marc C P; Hublitz, Philip; Schüle, Roland; M. Leonor Cancela; M. Leonor Cancela; M. Leonor Cancela; Nitschke, Yvonne; Pasch, Andreas; Quaglino, Daniela; Rutsch, Frank; Shanahan, Catherine; Sorribas, Victor; Szeri, Flora; Valdivielso, Pedro; Vanakker, Olivier; Kempf, Hervé

Natércia Conceição

Position

Researcher

Academic Degree

Doutoramento

Group Leader

Comparative, Adaptive and Functional Skeletal Biology

Group

Comparative, Adaptive and Functional Skeletal Biology

Professor Category

Assistant

Institution

Universidade do Algarve

Based at

Faro, Gambelas Campus, UAlg

Office Nr

2.76

Phone Nr

+351 289800057

Extension

7057

nconcei@ualg.pt

Keywords

zebrafish; extracellular matrix mineralization; cell culture; gene regulation; fish models of human diseases

CV DOWNLOAD

ORCID ID

Scopus

Organiza

Projects

Projects Coordination

	Title	Coordinator	Start	End	Financiamento	Budget CCMAR	Budget Total
IBIGEN-LHON	Investigação bigenómica translacional na Neuropatia Ótica Hereditária de Leber: Correlação Genótipo-Fenótipo	Natércia Conceição	04/2013	08/2015	FCT - Fundação para a Ciência e Tecnologia	7k€
GLARE	Padrão de expressão espaço-temporal da Matrix Gla Protein (MGP) e sua regulação durante o desenvolvimento em X. laevis.	Natércia Conceição	06/2005	06/2008	FCT - Fundação para a Ciência e Tecnologia	72k€

Projects Membership

	Title	Coordinator	Start	End	Budget CCMAR	Budget Total
SaMGP	Effect of extracellular calcium on MGP gene expression	Vincent Laizé	03/2003	03/2006	100k€	100k€

Activities

Type of Event

Other Events

MSc thesis presentation of Michele Loiodice

This Friday, the Master's student Michele Loiodice, will present and defend his thesis at the University of Algarve at 14:00 pm.

Group

Comparative, Adaptive and Functional Skeletal Biology

November 8, 2019

News

Experts discuss the skeleton during this week

CCMAR organizes this week the fifth conference “Interdisciplinary Approaches in Fish Skeletal Biology (IAFSB)” in Tavira, Portugal.

Publications

Year of Publication
2023	Varela D, Varela T, Conceição N, M. Cancela L. Regulation of human ZNF687, a gene associated with Paget's disease of bone. The International Journal of Biochemistry & Cell Biology. 2023;154:106332. doi:10.1016/j.biocel.2022.106332 Read more about Regulation of human ZNF687, a gene associated with Paget's disease of bone Google Scholar PT DOI
2023	Santos JMA, Laizé V, Gavaia PJ, Conceição N, M. Cancela L. Zebrafish Models to Study Ectopic Calcification and Calcium-Associated Pathologies. International Journal of Molecular Sciences. 2023;24(4):3366. doi:10.3390/ijms24043366 Read more about Zebrafish Models to Study Ectopic Calcification and Calcium-Associated Pathologies Google Scholar PT DOI
2023	Adrião A, Mariano S, Mariano J, et al. mef2ca and mef2cb Double Mutant Zebrafish Show Altered Craniofacial Phenotype and Motor Behaviour. Biomolecules. 2023;13(5):805. doi:10.3390/biom13050805 Read more about mef2ca and mef2cb Double Mutant Zebrafish Show Altered Craniofacial Phenotype and Motor Behaviour Google Scholar PT DOI
2022	Simão M, Conceição N, Imaginário S, Amaro J, Cancela MLeonor. Lab-it is taking molecular genetics to school. BioChem. 2022;2(2):160 - 170. doi:10.3390/biochem2020011 Read more about Lab-it is taking molecular genetics to school Google Scholar PT DOI
2022	Varela T, Varela D, Martins G, Conceição N, M. Cancela L. Cdkl5 mutant zebrafish shows skeletal and neuronal alterations mimicking human CDKL5 deficiency disorder. Scientific Reports. 2022;12(1). doi:10.1038/s41598-022-13364-1 Read more about Cdkl5 mutant zebrafish shows skeletal and neuronal alterations mimicking human CDKL5 deficiency disorder Google Scholar PT DOI
2022	Adrião A, Santana I, Ribeiro C, M. Cancela L, Conceição N, Grazina M. Identification of a novel mutation in MEF2C gene in an atypical patient with frontotemporal lobar degeneration. Neurological Sciences. 2022;43(1):319 - 326. doi:10.1007/s10072-021-05269-0 Read more about Identification of a novel mutation in MEF2C gene in an atypical patient with frontotemporal lobar degeneration Google Scholar PT DOI
2022	Silva IAL, Varela D, M. Cancela L, Conceição N. Zebrafish optineurin: genomic organization and transcription regulation. Genome. 2022;65(10):513 - 523. doi:10.1139/gen-2022-0019 Read more about Zebrafish optineurin: genomic organization and transcription regulation Google Scholar PT DOI
2021	Varela D, Varela T, Conceição N, et al. Functional analysis of two novel TBX5 variants present in individuals with Holt–Oram syndrome with different clinical manifestations. Molecular Genetics and Genomics. 2021;296(4):809 - 821. doi:10.1007/s00438-021-01781-2 Read more about Functional analysis of two novel TBX5 variants present in individuals with Holt–Oram syndrome with different clinical manifestations Google Scholar PT DOI
2021	Varela D, Conceição N, M. Cancela L. Transcriptional regulation of human T-box 5 gene (TBX5) by bone- and cardiac-related transcription factors. Gene. 2021;768:145322. doi:10.1016/j.gene.2020.145322 Read more about Transcriptional regulation of human T-box 5 gene (TBX5) by bone- and cardiac-related transcription factors Google Scholar PT DOI
2021	Cancela ML, Laizé V, Conceição N, Kempf H, Murshed M. Keutel syndrome, a review of 50 years of literature. Frontiers in Cell and Developmental Biology. 2021;9:642136. doi:10.3389/fcell.2021.642136 Read more about Keutel syndrome, a review of 50 years of literature Google Scholar PT DOI
2021	Varela T, Conceição N, Laizé V, Cancela ML. Transcriptional regulation of human DUSP4 gene by cancer‐related transcription factors. Journal of Cellular Biochemistry. 2021;122(10):1556 - 1566. doi:10.1002/jcb.v122.1010.1002/jcb.30078 Read more about Transcriptional regulation of human DUSP4 gene by cancer‐related transcription factors Google Scholar PT DOI
2020	Caiado H, Conceição N, Tiago D, et al. Evaluation of MGP gene expression in colorectal cancer. Gene. 2020;723:144120. doi:10.1016/j.gene.2019.144120 Read more about Evaluation of MGP gene expression in colorectal cancer Google Scholar PT DOI
2020	Caiado H, Conceição N, Tiago D, et al. Data on the evaluation of FGF2 gene expression in Colorectal Cancer. Data in Brief. 2020;31:105765. doi:10.1016/j.dib.2020.105765 Read more about Data on the evaluation of FGF2 gene expression in Colorectal Cancer Google Scholar PT DOI
2020	Varela T, Laizé V, Conceição N, et al. Expression of DUSP4 transcript variants as a potential biomarker for colorectal cancer. Biomarkers in Medicine. 2020;14(8):639 - 650. doi:10.2217/bmm-2019-0369 Read more about Expression of DUSP4 transcript variants as a potential biomarker for colorectal cancer Google Scholar PT DOI
2019	Bäck M, Aranyi T, M. Cancela L, et al. Endogenous Calcification Inhibitors in the Prevention of Vascular Calcification: A Consensus Statement From the COST Action EuroSoftCalcNet. Frontiers in Cardiovascular Medicine. 2019;5. doi:10.3389/fcvm.2018.00196 Read more about Endogenous Calcification Inhibitors in the Prevention of Vascular Calcification: A Consensus Statement From the COST Action EuroSoftCalcNet Google Scholar PT DOI
2018	Fazenda C, Martins G, Gavaia P, M. Cancela L, Conceição N. Generation of zebrafish Danio rerio (Hamilton, 1822) transgenic lines overexpressing a heat-shock mediated Gla-rich protein. Journal of Applied Ichthyology. 2018. doi:10.1111/jai.13662 Read more about Generation of zebrafish Danio rerio (Hamilton, 1822) transgenic lines overexpressing a heat-shock mediated Gla-rich protein Google Scholar DOI
2018	Silva IAL, Conceição N, Gagnon E, Brown JP, M. Cancela L, Michou L. Molecular effect of an OPTN common variant associated to Paget's disease of bone. PLOS ONE. 2018;13(5):e0197543. doi:10.1371/journal.pone.0197543.s007 Read more about Molecular effect of an OPTN common variant associated to Paget's disease of bone Google Scholar DOI
2018	Vitorino M, Cunha N, Conceição N, M. Cancela L. Expression pattern of cdkl5 during zebrafish early development: implications for use as model for atypical Rett syndrome. Molecular Biology Reports. 2018. doi:10.1007/s11033-018-4180-1 Read more about Expression pattern of cdkl5 during zebrafish early development: implications for use as model for atypical Rett syndrome Google Scholar DOI
2018	Vitorino M, Conceição N, M. Cancela L. Zebrafish (Danio rerio, Hamilton-Buchanan, 1822) as a model to study bone diseases associated with Rett syndrome. Journal of Applied Ichthyology. 2018;34(2):489 - 500. doi:10.1111/jai.13667 Read more about Zebrafish (Danio rerio, Hamilton-Buchanan, 1822) as a model to study bone diseases associated with Rett syndrome Google Scholar DOI
2018	Silva IAL, Conceição N, Gagnon E, et al. Effect of genetic variants of OPTN in the pathophysiology of Paget's disease of bone. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 2018;1864(1):143 - 151. doi:10.1016/j.bbadis.2017.10.008 Read more about Effect of genetic variants of OPTN in the pathophysiology of Paget's disease of bone Google Scholar DOI
2016	Tiago DM, Conceição N, Caiado H, Laizé V, M. Cancela L. Matrix Gla protein repression by miR-155 promotes oncogenic signals in breast cancer MCF-7 cells. FEBS Letters. 2016;590(8):1234-1241. doi:10.1002/1873-3468.12155 Read more about Matrix Gla protein repression by miR-155 promotes oncogenic signals in breast cancer MCF-7 cells Google Scholar PubMed DOI
2016	Adrião A, Conceição N, M. Cancela L. MEF2C orthologues from zebrafish: Evolution, expression and promoter regulation. Arch Biochem Biophys. 2016;591:43-56. doi:10.1016/j.abb.2015.12.004 Read more about MEF2C orthologues from zebrafish: Evolution, expression and promoter regulation. Google Scholar PubMed DOI
2016	Correia E, Conceição N, M. Cancela L, Belo JA. Matrix Gla Protein expression pattern in the early avian embryo. Int J Dev Biol. 2016;60(1-3):71-6. doi:10.1387/ijdb.150365jb Read more about Matrix Gla Protein expression pattern in the early avian embryo. Google Scholar PubMed DOI
2015	Simões B, Conceição N, Matias AC, Bragança J, Kelsh RN, M. Cancela L. Molecular characterization of cbfβ gene and identification of new transcription variants: implications for function. Arch Biochem Biophys. 2015;567:1-12. doi:10.1016/j.abb.2014.12.023 Read more about Molecular characterization of cbfβ gene and identification of new transcription variants: implications for function. Google Scholar PubMed DOI
2015	Fazenda C, Conceição N, M. Cancela L. Transcription factors from Sox family regulate expression of zebrafish Gla-rich protein 2 gene. Gene. 2015;572(1):57-62. doi:10.1016/j.gene.2015.06.079 Read more about Transcription factors from Sox family regulate expression of zebrafish Gla-rich protein 2 gene. Google Scholar PubMed DOI
2015	Silva IAL, Conceição N. Cloning, characterization and analysis of the 5′ regulatory region of zebrafish xpd gene. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 2015;185:47 - 53. doi:10.1016/j.cbpb.2015.04.003 Read more about Cloning, characterization and analysis of the 5′ regulatory region of zebrafish xpd gene Google Scholar DOI
2014	Silva IAL, Conceição N, Michou L, M. Cancela L. Can zebrafish be a valid model to study Paget's disease of bone?. Journal of Applied Ichthyology. 2014;30(4):678 - 688. doi:10.1111/jai.2014.30.issue-410.1111/jai.12523 Read more about Can zebrafish be a valid model to study Paget's disease of bone? Google Scholar DOI
2014	Silva IAL, Cox CJ, Leite RB, M. Cancela L, Conceição N. Evolutionary conservation of TFIIH subunits: implications for the use of zebrafish as a model to study TFIIH function and regulation. Comp Biochem Physiol B Biochem Mol Biol. 2014;172-173:9-20. doi:10.1016/j.cbpb.2014.03.007 Read more about Evolutionary conservation of TFIIH subunits: implications for the use of zebrafish as a model to study TFIIH function and regulation. Google Scholar PubMed DOI
2014	M. Cancela L, Laizé V, Conceição N. Matrix Gla protein and osteocalcin: from gene duplication to neofunctionalization. Arch Biochem Biophys. 2014;561:56-63. doi:10.1016/j.abb.2014.07.020 Read more about Matrix Gla protein and osteocalcin: from gene duplication to neofunctionalization. Google Scholar PubMed DOI
2014	Simões B, Conceição N, Kelsh RN, M. Cancela L. Identification of cis -regulatory elements in the upstream regions of zebrafish runx3 through in silico analysis: implications for function. Journal of Applied Ichthyology. 2014;30(4):661 - 670. doi:10.1111/jai.2014.30.issue-410.1111/jai.12536 Read more about Identification of cis -regulatory elements in the upstream regions of zebrafish runx3 through in silico analysis: implications for function Google Scholar DOI
2013	Conceição N, Viegas M, Fidalgo J, M. Cancela L. Development and characterization of Xl1, a Xenopus laevis chondrocyte-like cell culture. Mol Cell Biochem. 2013;373(1-2):41-51. doi:10.1007/s11010-012-1473-x Read more about Development and characterization of Xl1, a Xenopus laevis chondrocyte-like cell culture. Google Scholar PubMed DOI
2012	Conceição N, Fazenda C, M. Cancela L. Comparative gene promoter analysis: an in silico strategy to identify candidate regulatory factors for Gla Rich Protein. Journal of Applied Ichthyology. 2012;28(3):372 - 376. doi:10.1111/j.1439-0426.2012.02001.x Read more about Comparative gene promoter analysis: an in silico strategy to identify candidate regulatory factors for Gla Rich Protein Google Scholar DOI
2012	Fazenda C, Silva IAL, M. Cancela L, Conceição N. Molecular characterization of two paralog genes encoding Gla-rich protein (Grp) in zebrafish. Journal of Applied Ichthyology. 2012;28(3):377 - 381. doi:10.1111/j.1439-0426.2012.02004.x Read more about Molecular characterization of two paralog genes encoding Gla-rich protein (Grp) in zebrafish Google Scholar DOI
2012	Michou L, Conceição N, Morissette J, et al. Genetic association study of UCMA/GRP and OPTN genes (PDB6 locus) with Paget's disease of bone. Bone. 2012;51(4):720-8. doi:10.1016/j.bone.2012.06.028 Read more about Genetic association study of UCMA/GRP and OPTN genes (PDB6 locus) with Paget's disease of bone. Google Scholar PubMed DOI
2012	M. Cancela L, Conceição N, Laizé V. Gla-rich protein, a new player in tissue calcification?. Adv Nutr. 2012;3(2):174-81. doi:10.3945/an.111.001685 Read more about Gla-rich protein, a new player in tissue calcification? Google Scholar PubMed DOI
2012	Silva IAL, M. Cancela L, Conceição N. Molecular cloning and expression analysis of xpd from zebrafish (Danio rerio). Mol Biol Rep. 2012;39(5):5339-48. doi:10.1007/s11033-011-1333-x Read more about Molecular cloning and expression analysis of xpd from zebrafish (Danio rerio). Google Scholar PubMed DOI
2010	Fazenda C, Simões B, Kelsh RN, M. Cancela L, Conceição N. Dual transcriptional regulation by runx2 of matrix Gla protein in Xenopus laevis. Gene. 2010;450(1-2):94-102. doi:10.1016/j.gene.2009.10.007 Read more about Dual transcriptional regulation by runx2 of matrix Gla protein in Xenopus laevis. Google Scholar PubMed DOI
2010	Conceição N, Cox CJ, Simões B, Viegas M, M. Cancela L. Comparative promoter analysis and its application to the identification of candidate regulatory factors of cartilage-expressed genes. Journal of Applied Ichthyology. 2010;26(2):245 - 250. doi:10.1111/jai.2010.26.issue-210.1111/j.1439-0426.2010.01415.x Read more about Comparative promoter analysis and its application to the identification of candidate regulatory factors of cartilage-expressed genes Google Scholar DOI
2008	Conceição N, Laizé V, Simões B, Pombinho AR, M. Cancela L. Retinoic acid is a negative regulator of matrix Gla protein gene expression in teleost fish Sparus aurata. Biochim Biophys Acta. 2008;1779(1):28-39. doi:10.1016/j.bbagrm.2007.11.003 Read more about Retinoic acid is a negative regulator of matrix Gla protein gene expression in teleost fish Sparus aurata. Google Scholar PubMed DOI
2006	Simões B, Conceição N, Viegas CSB, et al. Identification of a promoter element within the zebrafish colXalpha1 gene responsive to runx2 isoforms Osf2/Cbfa1 and til-1 but not to pebp2alphaA2. Calcif Tissue Int. 2006;79(4):230-44. doi:10.1007/s00223-006-0111-6 Read more about Identification of a promoter element within the zebrafish colXalpha1 gene responsive to runx2 isoforms Osf2/Cbfa1 and til-1 but not to pebp2alphaA2. Google Scholar PubMed DOI
2005	Pinto JP, Conceição NM, Viegas CSB, et al. Identification of a new pebp2alphaA2 isoform from zebrafish runx2 capable of inducing osteocalcin gene expression in vitro. J Bone Miner Res. 2005;20(8):1440-53. doi:10.1359/JBMR.050318 Read more about Identification of a new pebp2alphaA2 isoform from zebrafish runx2 capable of inducing osteocalcin gene expression in vitro. Google Scholar PubMed DOI
2005	Conceição N, Silva AC, Fidalgo J, Belo JA, M. Cancela L. Identification of alternative promoter usage for the matrix Gla protein gene. Evidence for differential expression during early development in Xenopus laevis. FEBS J. 2005;272(6):1501-10. doi:10.1111/j.1742-4658.2005.04590.x Read more about Identification of alternative promoter usage for the matrix Gla protein gene. Evidence for differential expression during early development in Xenopus laevis. Google Scholar PubMed DOI
2003	Pinto JP, Conceição N, Gavaia P, M. Cancela L. Matrix Gla protein gene expression and protein accumulation colocalize with cartilage distribution during development of the teleost fish Sparus aurata. Bone. 2003;32(3):201-10. Read more about Matrix Gla protein gene expression and protein accumulation colocalize with cartilage distribution during development of the teleost fish Sparus aurata. Google Scholar PubMed
2002	Conceição N, Henriques NM, Ohresser MCP, Hublitz P, Schüle R, M. Cancela L. Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis. Functional analysis of the promoter identifies a calcium sensitive region required for basal activity. Eur J Biochem. 2002;269(7):1947-56. Read more about Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis. Functional analysis of the promoter identifies a calcium sensitive region required for basal activity. Google Scholar PubMed

Supervising

Andreia Lúcia Gonçalves Adrião

Iris Alexandra Lopes da Silva

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