Cellular and humoral immune responses of Senegalese sole, Solea senegalensis (Kaup), following challenge with two Photobacterium damselae subsp. piscicida strains from different geographical origins. | - CCMAR -

Journal Article

TítuloCellular and humoral immune responses of Senegalese sole, Solea senegalensis (Kaup), following challenge with two Photobacterium damselae subsp. piscicida strains from different geographical origins.
Publication TypeJournal Article
AuthorsCostas, B, Rêgo, PCNP, Simões, I, Marques, JF, Castro-Cunha, M, Afonso, A
Year of Publication2013
JournalJ Fish Dis
Volume36
Questão6
Date Published2013 Jun
Pagination543-53
ISSN1365-2761
Palavras-chaveAnimals, Europe, Fish Diseases, Flatfishes, Gram-Negative Bacterial Infections, Immunity, Cellular, Immunity, Humoral, Japan, Leukocytes, Photobacterium, Virulence
Abstract

The present study aimed to investigate leucocyte responses to inflammation as well as some innate immune parameters of Senegalese sole, Solea senegalensis, following challenge with two strains of Photobacterium damselae subsp. piscicida belonging to the European and Japanese clones described for this bacterium. Pathogenicity assays were performed to assess the virulence of each Photobacterium damselae subsp. piscicida strain for sole. Subsequently, fish were intraperitoneally injected with phosphate-buffered saline (control) or two concentrations (2 × 10² and 2 × 10⁶ CFU mL⁻¹) of each bacterial strain and sampled after 6 and 24 h. Results showed that the European isolate induces a higher degree of response than the Japanese strain. While blood neutrophilia and monocytosis correlated well with the increase in neutrophil and macrophage numbers in the peritoneal cavity, fish infected with the European isolate presented higher peritoneal cell numbers than fish challenged with the Japanese strain. In addition, alternative complement pathway activity and respiratory burst of head kidney leucocytes increased significantly in fish infected with the European isolate. The enhanced innate immune response displayed by Senegalese sole challenged with the European isolate is probably due to the higher degree of virulence presented by this Photobacterium damselae subsp. piscicida strain.

DOI10.1111/jfd.12033
Sapientia

http://www.ncbi.nlm.nih.gov/pubmed/23163607?dopt=Abstract

Alternate JournalJ. Fish Dis.
PubMed ID23163607
CCMAR Authors