Journal Article
Título | Intra-adrenal interactions in fish: catecholamine stimulated cortisol release in sea bass (Dicentrarchus labrax L.). |
Publication Type | Journal Article |
Authors | Rotllant, J, Ruane, NM, Dinis, MT, Canario, AVM, Power, DM |
Year of Publication | 2006 |
Journal | Comp Biochem Physiol A Mol Integr Physiol |
Volume | 143 |
Questão | 3 |
Date Published | 2006 Mar |
Pagination | 375-81 |
ISSN | 1095-6433 |
Palavras-chave | Adrenergic alpha-Antagonists, Adrenergic beta-Antagonists, Animals, Bass, Epinephrine, Hydrocortisone, Inositol, Interrenal Gland, Norepinephrine, Prazosin, Second Messenger Systems, Sotalol |
Abstract | The effect of the catecholamines, adrenaline and noradrenaline, on sea bass (Dicentrarchus labrax) and sea bream (Sparus auratus) interrenal cortisol production was studied in vitro using a dynamic superfusion system technique. Increasing concentrations of catecholamines (10(-6), 10(-8) and 10(-10) M) stimulated cortisol production in a dose-dependent manner, in sea bass only. The increase in cortisol production stimulated by adrenaline (10(-6) M) and noradrenaline (10(-6) M) was inhibited by sotalol (2 x 10(-5) M), but not by prazosin suggesting that catecholamines stimulate cortisol release through the beta-receptor subtype. To evaluate catecholamine-induced signal transduction in head kidney cells, measurements of cAMP production and [H3]myo-inositol incorporation were determined in head kidney cell suspensions. Adrenaline and noradrenaline (10(-6) M) increased cAMP production, but had no effect on total inositol phosphate accumulation. These results indicate that catecholamines released from the chromaffin cells within the interrenal tissue may act as a paracrine factor to stimulate interrenal steroidogenesis in the sea bass. |
DOI | 10.1016/j.cbpa.2005.12.027 |
Sapientia | |
Alternate Journal | Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. |
PubMed ID | 16443381 |