Molecular richness and biotechnological potential of bacteria cultured from Irciniidae sponges in the north-east Atlantic. | - CCMAR -

Journal Article

TítuloMolecular richness and biotechnological potential of bacteria cultured from Irciniidae sponges in the north-east Atlantic.
Publication TypeJournal Article
AuthorsEsteves, AIS, Hardoim, CCP, Xavier, JR, Gonçalves, JMS, Costa, R
Year of Publication2013
JournalFEMS Microbiol Ecol
Volume85
Questão3
Date Published2013 Sep
Pagination519-36
ISSN1574-6941
Palavras-chaveAnimals, Antibiosis, Atlantic Ocean, Bacteria, Biodiversity, Biotechnology, Genotype, Peptide Synthases, Phylogeny, Polyketide Synthases, Porifera, Rhodobacteraceae, RNA, Ribosomal, 16S
Abstract

Several bioactive compounds originally isolated from marine sponges have been later ascribed or suggested to be synthesized by their symbionts. The cultivation of sponge-associated bacteria provides one possible route to the discovery of these metabolites. Here, we determine the bacterial richness cultured from two irciniid sponge species, Sarcotragus spinosulus and Ircinia variabilis, and ascertain their biotechnological potential. A total of 279 isolates were identified from 13 sponge specimens. These were classified into 17 genera - with Pseudovibrio, Ruegeria and Vibrio as the most dominant - and 3 to 10 putatively new bacterial species. While 16S rRNA gene sequencing identified 29 bacterial phylotypes at the 'species' level (97% sequence similarity), whole-genome BOX-PCR fingerprinting uncovered 155 genotypes, unveiling patterns of specimen-dependent occurrence of prevailing bacterial genomes across sponge individuals. Among the BOX-PCR genotypes recovered, 34% were active against clinically relevant strains, with Vibrio isolates producing the most active antagonistic effect. Several Pseudovibrio genotypes showed the presence of polyketide synthase (PKS) genes, and these were for the first time detected in isolates of the genus Aquimarina (Bacteroidetes). Our results highlight great biotechnological potential and interest for the Irciniidae sponge family and their diversified bacterial genomes.

DOI10.1111/1574-6941.12140
Sapientia

http://www.ncbi.nlm.nih.gov/pubmed/23621863?dopt=Abstract

Alternate JournalFEMS Microbiol. Ecol.
PubMed ID23621863