Journal Article
Título | Olfactory transduction pathways in the Senegalese sole Solea senegalensis. |
Publication Type | Journal Article |
Authors | Velez, Z, Hubbard, PC, Barata, EN, Canario, AVM |
Year of Publication | 2013 |
Journal | J Fish Biol |
Volume | 83 |
Questão | 3 |
Date Published | 2013 Sep |
Pagination | 501-14 |
ISSN | 1095-8649 |
Palavras-chave | Adenine, Adenylyl Cyclase Inhibitors, Adenylyl Cyclases, Amino Acids, Animals, Bile Acids and Salts, Cyclic AMP, Epithelium, Estrenes, Flatfishes, Pyrrolidinones, Receptors, Odorant, Signal Transduction, Smell, Type C Phospholipases |
Abstract | This study tested whether differences in sensitivity between the upper and lower olfactory epithelia of Solea senegalensis are associated with different odorant receptors and transduction pathways, using the electro-olfactogram. Receptor mechanisms were assessed by cross-adaptation with amino acids (L-cysteine, L-phenylalanine and 1-methyl-L-tryptophan) and bile acids (taurocholic acid and cholic acid). This suggested that relatively specific receptors exist for 1-methyl-L-tryptophan and L-phenylalanine (food-related odorants) in the lower epithelium, and for taurocholic acid (conspecific-derived odorant) in the upper. Inhibition by U73122 [a phospholipase C (PLC) inhibitor] suggested that olfactory responses to amino acids were mediated mostly, but not entirely, by PLC-mediated transduction (IC50 ; 15-55 nM), whereas bile acid responses were mediated by both PLC and adenylate cyclase-cyclic adenosine monophosphate (AC-cAMP) (using SQ-22536; an AC inhibitor). Simultaneous application of both drugs rarely inhibited responses completely, suggesting possible involvement of non-PLC and non-AC mediated mechanisms. For aromatic amino acids and bile acids, there were differences in the contribution of each transduction pathway (PLC, AC and non-PLC and non-AC) between the two epithelia. These results suggest that differences in sensitivity of the two epithelia are associated with differences in odorant receptors and transduction mechanisms. |
DOI | 10.1111/jfb.12185 |
Sapientia | |
Alternate Journal | J. Fish Biol. |
PubMed ID | 23991870 |