In vitro characterization of acid secretion in the gilthead sea bream (Sparus aurata) stomach. | - CCMAR -

Journal Article

TítuloIn vitro characterization of acid secretion in the gilthead sea bream (Sparus aurata) stomach.
Publication TypeJournal Article
AuthorsMárquez, L, Fuentes, J
Year of Publication2014
JournalComp Biochem Physiol A Mol Integr Physiol
Volume167
Date Published2014 Jan
Pagination52-8
ISSN1531-4332
Palavras-chave1-Methyl-3-isobutylxanthine, Animals, Carbachol, Colforsin, Cyclic AMP, Electric Impedance, Gadolinium, Gastric Acid, Gastric Mucosa, In Vitro Techniques, Macrolides, Muscarinic Agonists, Omeprazole, Proton Pump Inhibitors, Receptors, Calcium-Sensing, Sea Bream
Abstract

The gastric acid secretion of juvenile Sparus aurata was characterized in Ussing chambers; secretion rates were determined by a pH-stat method at pH5.50 and bioelectrical parameters were measured in current-clamped tissues. The basal secretion equaled to 535±87nmol·cm(-2)·h(-1). Serosal carbachol 100μM produced an increase (ΔJH(+)) of 725±133nmol·cm(-2)·h(-1) from basal secretion, this effect being inhibited by mucosal omeprazole 100μM. Basal secretion was also sensitive to the combination of serosal forskolin (FK) 10μM+serosal isobutylmethylxanthine (IBMX) 100μM (ΔJH(+)=793±239nmol·cm(-2)·h(-1)); this effect was insensitive to mucosal omeprazole 100mM but inhibited by mucosal bafilomycin A1 100nM. The effect of carbachol proceeded within a few minutes (<10min), whereas the effect of FK+IBMX was gradual, taking 40min to reach the maximum. The addition of mucosal gadolinium (Gd(3+)) 100μM, a potent calcium-sensing receptor (CaR) agonist, stimulated the basal secretion (ΔJH(+)=340±81nmol·cm(-2)·h(-1)). The present results indicate that the acid secretion mechanism in the sea bream stomach is regulated by muscarinic and CaR-like receptors, cAMP is implicated in the signal transduction, and at least two proton pumps, a HK-ATPase and a V-ATPase contribute to acid secretion.

DOI10.1016/j.cbpa.2013.10.016
Sapientia

http://www.ncbi.nlm.nih.gov/pubmed/24126049?dopt=Abstract

Alternate JournalComp. Biochem. Physiol., Part A Mol. Integr. Physiol.
PubMed ID24126049
CCMAR Authors