Journal Article
| Title | Characterization of a sea bream (Sparus aurata) thyroid hormone receptor-beta clone expressed during embryonic and larval development. |
| Publication Type | Journal Article |
| Authors | Nowell, MA, Power, DM, Canario, AVM, Llewellyn, L, Sweeney, GE |
| Year of Publication | 2001 |
| Journal | Gen Comp Endocrinol |
| Volume | 123 |
| Issue | 1 |
| Date Published | 2001 Jul |
| Pagination | 80-9 |
| ISSN | 0016-6480 |
| Keywords | Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, DNA, Complementary, Electrophoresis, Polyacrylamide Gel, Embryonic Development, Female, Gene Expression, Gene Library, Larva, Molecular Sequence Data, Ovary, Receptors, Thyroid Hormone, Reverse Transcriptase Polymerase Chain Reaction, Sea Bream, Sequence Alignment, Thyroxine, Triiodothyronine |
| Abstract | A clone encoding thyroid hormone receptor-beta (TR-beta) was isolated from a sea bream (Sparus aurata) ovary cDNA library. Sea bream (sb)TR-beta is closely related to its counterparts from other vertebrates and, like them, preferentially binds T3 rather than T4. However, the putative sbTR-beta protein contains a nine-amino-acid insert that is also present in the corresponding proteins from flounder and salmon but absent in TR-betas from zebrafish and terrestrial vertebrates. Semiquantitative RT-PCR analysis showed that sbTR-beta transcripts begin to accumulate during gastrulation and increase markedly in quantity up to the period around hatch (ca. 40 h postfertilization) before declining slightly. In adult tissues, TR-beta mRNA was present in approximately equal quantities in heart, intestine, brain, kidney, skeletal muscle, liver, and gill. The significance of the relatively strong expression of TR-beta during sea bream embryogenesis is discussed. |
| DOI | 10.1006/gcen.2001.7649 |
| Sapientia | |
| Alternate Journal | Gen. Comp. Endocrinol. |
| PubMed ID | 11551120 |
