Identification of a new cartilage-specific S100-like protein up-regulated during endo/perichondral mineralization in gilthead seabream. | - CCMAR -

Journal Article

TitleIdentification of a new cartilage-specific S100-like protein up-regulated during endo/perichondral mineralization in gilthead seabream.
Publication TypeJournal Article
AuthorsFonseca, VG, Rosa, J, Laizé, V, Gavaia, P, M. Cancela, L
Year of Publication2011
JournalGene Expr Patterns
Volume11
Issue7
Date Published2011 Oct
Pagination448-55
ISSN1872-7298
KeywordsAmino Acid Sequence, Animals, Base Sequence, Calcification, Physiologic, Cartilage, Cell Line, Chondrocytes, Computational Biology, EF Hand Motifs, Gene Expression Regulation, Developmental, Molecular Sequence Data, Osteogenesis, Phylogeny, S100 Proteins, Sea Bream
Abstract

Calcium ions and calcium-binding proteins play a major role in many cellular processes, in particular skeletogenesis and bone formation. We report here the discovery of a novel S100 protein in fish and the analysis of its gene expression patterns. A 648-bp full-length cDNA encoding an 86-amino acid S100-like calcium-binding protein was identified through the subtractive hybridization of a gilthead seabream (Sparus aurata) cDNA library constructed to identify genes associated with in vitro mineralization. Deduced protein lacks an identifiable signal peptide and exhibits two EF-hand motifs characteristic of S100 proteins. Phylogenetic and bioinformatic analyses of S100 sequences suggested that gilthead seabream protein represents a novel and fish-specific member of the S100 protein family. Expression of S100-like gene was up-regulated during the in vitro mineralization of bone-derived cell lines and during seabream development, from larvae throughout adulthood, reflecting skeletogenesis. Restriction of S100-like gene expression to chondrocytes of cartilaginous tissues undergoing endo/perichondral mineralization in juvenile fish further confirmed the mineralogenic role of the protein in fish and emphasized the potential of S100-like as a marker of mineralizing cartilage in developing fish.

DOI10.1016/j.gep.2011.07.003
Sapientia

http://www.ncbi.nlm.nih.gov/pubmed/21803172?dopt=Abstract

Alternate JournalGene Expr. Patterns
PubMed ID21803172