Journal Article
Title | Identification of a new cartilage-specific S100-like protein up-regulated during endo/perichondral mineralization in gilthead seabream. |
Publication Type | Journal Article |
Authors | Fonseca, VG, Rosa, J, Laizé, V, Gavaia, P, M. Cancela, L |
Year of Publication | 2011 |
Journal | Gene Expr Patterns |
Volume | 11 |
Issue | 7 |
Date Published | 2011 Oct |
Pagination | 448-55 |
ISSN | 1872-7298 |
Keywords | Amino Acid Sequence, Animals, Base Sequence, Calcification, Physiologic, Cartilage, Cell Line, Chondrocytes, Computational Biology, EF Hand Motifs, Gene Expression Regulation, Developmental, Molecular Sequence Data, Osteogenesis, Phylogeny, S100 Proteins, Sea Bream |
Abstract | Calcium ions and calcium-binding proteins play a major role in many cellular processes, in particular skeletogenesis and bone formation. We report here the discovery of a novel S100 protein in fish and the analysis of its gene expression patterns. A 648-bp full-length cDNA encoding an 86-amino acid S100-like calcium-binding protein was identified through the subtractive hybridization of a gilthead seabream (Sparus aurata) cDNA library constructed to identify genes associated with in vitro mineralization. Deduced protein lacks an identifiable signal peptide and exhibits two EF-hand motifs characteristic of S100 proteins. Phylogenetic and bioinformatic analyses of S100 sequences suggested that gilthead seabream protein represents a novel and fish-specific member of the S100 protein family. Expression of S100-like gene was up-regulated during the in vitro mineralization of bone-derived cell lines and during seabream development, from larvae throughout adulthood, reflecting skeletogenesis. Restriction of S100-like gene expression to chondrocytes of cartilaginous tissues undergoing endo/perichondral mineralization in juvenile fish further confirmed the mineralogenic role of the protein in fish and emphasized the potential of S100-like as a marker of mineralizing cartilage in developing fish. |
DOI | 10.1016/j.gep.2011.07.003 |
Sapientia | |
Alternate Journal | Gene Expr. Patterns |
PubMed ID | 21803172 |