Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae. | - CCMAR -

Journal Article

TitleMicroplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae.
Publication TypeJournal Article
AuthorsPereira, H, Barreira, L, Mozes, A, Florindo, C, Polo, C, Duarte, CV, Custódio, L, Varela, J
Year of Publication2011
JournalBiotechnol Biofuels
Volume4
Issue1
Date Published2011 Dec 22
Pagination61
ISSN1754-6834
Abstract

We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the growth of the same cultures in liquid medium. This approach enabled us to isolate a biodiverse collection of several axenic and unialgal cultures of different phyla.
DOI10.1186/1754-6834-4-61
Sapientia

http://www.ncbi.nlm.nih.gov/pubmed/22192119?dopt=Abstract
Alternate JournalBiotechnol Biofuels
PubMed ID22192119
PubMed Central IDPMC3287250
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