Identification of proteins with potential osteogenic activity present in the water-soluble matrix proteins from Crassostrea gigas nacre using a proteomic approach. | - CCMAR -

Journal Article

TitleIdentification of proteins with potential osteogenic activity present in the water-soluble matrix proteins from Crassostrea gigas nacre using a proteomic approach.
Publication TypeJournal Article
AuthorsOliveira, DV, Silva, TS, Cordeiro, OD, Cavaco, SI, Simes, DC
Year of Publication2012
JournalScientificWorldJournal
Volume2012
Date Published2012
Pagination765909
ISSN1537-744X
KeywordsAnimals, Chromatography, Liquid, Crassostrea, Electrophoresis, Gel, Two-Dimensional, Osteogenesis, Proteins, Proteomics, Solubility, Tandem Mass Spectrometry, Water
Abstract

Nacre, when implanted in vivo in bones of dogs, sheep, mice, and humans, induces a biological response that includes integration and osteogenic activity on the host tissue that seems to be activated by a set of proteins present in the nacre water-soluble matrix (WSM). We describe here an experimental approach that can accurately identify the proteins present in the WSM of shell mollusk nacre. Four proteins (three gigasin-2 isoforms and a cystatin A2) were for the first time identified in WSM of Crassostrea gigas nacre using 2DE and LC-MS/MS for protein identification. These proteins are thought to be involved in bone remodeling processes and could be responsible for the biocompatibility shown between bone and nacre grafts. These results represent a contribution to the study of shell biomineralization process and opens new perspectives for the development of new nacre biomaterials for orthopedic applications.

DOI10.1100/2012/765909
Sapientia

http://www.ncbi.nlm.nih.gov/pubmed/22666151?dopt=Abstract

Alternate JournalScientificWorldJournal
PubMed ID22666151
PubMed Central IDPMC3361287
CCMAR Authors