Journal Article
Título | Identification of proteins with potential osteogenic activity present in the water-soluble matrix proteins from Crassostrea gigas nacre using a proteomic approach. |
Publication Type | Journal Article |
Authors | Oliveira, DV, Silva, TS, Cordeiro, OD, Cavaco, SI, Simes, DC |
Year of Publication | 2012 |
Journal | ScientificWorldJournal |
Volume | 2012 |
Date Published | 2012 |
Pagination | 765909 |
ISSN | 1537-744X |
Palavras-chave | Animals, Chromatography, Liquid, Crassostrea, Electrophoresis, Gel, Two-Dimensional, Osteogenesis, Proteins, Proteomics, Solubility, Tandem Mass Spectrometry, Water |
Abstract | Nacre, when implanted in vivo in bones of dogs, sheep, mice, and humans, induces a biological response that includes integration and osteogenic activity on the host tissue that seems to be activated by a set of proteins present in the nacre water-soluble matrix (WSM). We describe here an experimental approach that can accurately identify the proteins present in the WSM of shell mollusk nacre. Four proteins (three gigasin-2 isoforms and a cystatin A2) were for the first time identified in WSM of Crassostrea gigas nacre using 2DE and LC-MS/MS for protein identification. These proteins are thought to be involved in bone remodeling processes and could be responsible for the biocompatibility shown between bone and nacre grafts. These results represent a contribution to the study of shell biomineralization process and opens new perspectives for the development of new nacre biomaterials for orthopedic applications. |
DOI | 10.1100/2012/765909 |
Sapientia | |
Alternate Journal | ScientificWorldJournal |
PubMed ID | 22666151 |
PubMed Central ID | PMC3361287 |