Overexpression of four and a half LIM domains protein 2 promotes epithelial-mesenchymal transition-like phenotype in fish pre-osteoblasts. | - CCMAR -

Journal Article

TitleOverexpression of four and a half LIM domains protein 2 promotes epithelial-mesenchymal transition-like phenotype in fish pre-osteoblasts.
Publication TypeJournal Article
AuthorsRafael, MS, Laizé, V, Florindo, C, Ferraresso, S, Bargelloni, L, M. Cancela, L
Year of Publication2012
JournalBiochimie
Volume94
Issue5
Date Published2012 May
Pagination1128-34
ISSN1638-6183
KeywordsAlkaline Phosphatase, Animals, Blotting, Western, Cell Line, Cell Proliferation, Collagen, Epithelial-Mesenchymal Transition, Glycosaminoglycans, Real-Time Polymerase Chain Reaction, Sea Bream, Transcription Factors
Abstract

FHL2 is a multifunctional protein involved in gene transcription regulation and cytoarchitecture modulation that has been recently associated with epithelial-mesenchymal transition (EMT) in colon cancer. Overexpression of FHL2 in a fish pre-osteoblastic cell line promoted cell dedifferentiation and impaired its extracellular matrix mineralization capacity. Cell cultures also acquired a novel three-dimensional structure organization, their proliferation rate was enhanced and gene expression profile was altered in agreement with an EMT-like phenotype upon overexpression of FHL2. Altogether, our results provide additional support to the relevance of FHL2 for cell differentiation and its association with hallmarks of cancer phenotype.
DOI10.1016/j.biochi.2012.01.013
Sapientia

http://www.ncbi.nlm.nih.gov/pubmed/22285966?dopt=Abstract
Alternate JournalBiochimie
PubMed ID22285966
CCMAR Authors
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