Journal Article
| Título | Overexpression of four and a half LIM domains protein 2 promotes epithelial-mesenchymal transition-like phenotype in fish pre-osteoblasts. |
| Publication Type | Journal Article |
| Authors | Rafael, MS, Laizé, V, Florindo, C, Ferraresso, S, Bargelloni, L, M. Cancela, L |
| Year of Publication | 2012 |
| Journal | Biochimie |
| Volume | 94 |
| Questão | 5 |
| Date Published | 2012 May |
| Pagination | 1128-34 |
| ISSN | 1638-6183 |
| Palavras-chave | Alkaline Phosphatase, Animals, Blotting, Western, Cell Line, Cell Proliferation, Collagen, Epithelial-Mesenchymal Transition, Glycosaminoglycans, Real-Time Polymerase Chain Reaction, Sea Bream, Transcription Factors |
| Abstract | FHL2 is a multifunctional protein involved in gene transcription regulation and cytoarchitecture modulation that has been recently associated with epithelial-mesenchymal transition (EMT) in colon cancer. Overexpression of FHL2 in a fish pre-osteoblastic cell line promoted cell dedifferentiation and impaired its extracellular matrix mineralization capacity. Cell cultures also acquired a novel three-dimensional structure organization, their proliferation rate was enhanced and gene expression profile was altered in agreement with an EMT-like phenotype upon overexpression of FHL2. Altogether, our results provide additional support to the relevance of FHL2 for cell differentiation and its association with hallmarks of cancer phenotype. |
| DOI | 10.1016/j.biochi.2012.01.013 |
| Sapientia | |
| Alternate Journal | Biochimie |
| PubMed ID | 22285966 |
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