Calcium-transport in quiescent and 1,25-dihydroxycholecalciferol-stimulated human osteosarcoma cells. Role in 24 hydroxylase enhancement. | - CCMAR -

Journal Article

TítuloCalcium-transport in quiescent and 1,25-dihydroxycholecalciferol-stimulated human osteosarcoma cells. Role in 24 hydroxylase enhancement.
Publication TypeJournal Article
AuthorsRebut-Bonneton, C, Ly, SY, M. Cancela, L
Year of Publication1988
JournalHorm Metab Res
Volume20
Questão6
Date Published1988 Jun
Pagination339-43
ISSN0018-5043
Palavras-chaveCalcitriol, Calcium, Cycloheximide, Cytochrome P-450 Enzyme System, Humans, Nicardipine, Osteosarcoma, Protein Biosynthesis, Proteins, Steroid Hydroxylases, Tumor Cells, Cultured, Vitamin D3 24-Hydroxylase
Abstract

The present study evaluates in osteosarcoma cells, the effects of a calcium channel inhibitor nicardipine in 24-hydroxylase activity and 45Ca desaturation curve in presence of 1,25-dihydroxycholecalciferol (1,25(OH)2D3). This sterol induced an increase in 24-OHase activity and 45Ca fluxes. Nicardipine reversed the effect of 1,25(OH)2D3 on 45Ca fluxes but reinforced the enhancement of the 24-OHase activity. The fact that the effects of 1,25(OH)2D3 were reduced by cycloheximide support the hypothesis of a de novo protein synthesis. Our study has allowed us to dissociate the effects of 1,25(OH)2D3 on 24-OHase enhancement from those on Ca2+ transport.

DOI10.1055/s-2007-1010831
Sapientia

http://www.ncbi.nlm.nih.gov/pubmed/3166448?dopt=Abstract

Alternate JournalHorm. Metab. Res.
PubMed ID3166448
CCMAR Authors