Evolution of matrix and bone  gamma-carboxyglutamic acid proteins in vertebrates

Vincent Laizé; Martel, Paulo; Carla SB Viegas; Price, Paul A.; M. Leonor Cancela; Bargelloni, Luca; Boudry, Pierre; Boulo, Viviane; Dias, Jorge; Huvet, Arnaud; Vincent Laizé; Lapègue, Sylvie; Leite, Ricardo; Mira, Sara; Nielsen, Einar E.; Planas, Josep V.; Roher, Nerea; Sarropoulou, Elena; Volckaert, Filip A.M.

Vincent Laizé

Position

Senior Researcher

Academic Degree

Doutoramento

Group Leader

Comparative, Adaptive and Functional Skeletal Biology

Group

Comparative, Adaptive and Functional Skeletal Biology

Based at

Faro, Gambelas Campus, UAlg

Office Nr

2.76

Phone Nr

+351 289800057

Extension

7057

vlaize@ualg.pt

Keywords

zebrafish, extracellular matrix mineralization, bone development, marine biotechnology, osteotoxicity, gene regulation, regeneration

ORCID ID

ResearchGate

Scopus

Organiza

Main Info

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For more information see also the webpage of the BIOSKEL lab at http://bioskel.ccmar.ualg.pt/

Projects

Projects Coordination

	Title	Coordinator	Start	End	Financiamento	Budget CCMAR	Budget Total
DEEPBONE	DEEPBONE - Biologia do osso de peixes de profundidade	Vincent Laizé	01/2012	06/2015	FCT - Fundação para a Ciência e Tecnologia	109k€	109k€
AQUATOX	Avaliação da toxidade óssea de poluentes aquáticos.	Vincent Laizé	07/2011	12/2014	FCT - Fundação para a Ciência e Tecnologia	113k€	113k€
SaMGP	Effect of extracellular calcium on MGP gene expression	Vincent Laizé	03/2003	03/2006		100k€	100k€

Projects Membership

	Title	Coordinator	Start	End	Budget CCMAR	Budget Total
GERMROS	The imprinting characteristics of spermatogonia antioxidant capacity and their role in oxidative stress tolerance	Elvira Fernandez	01/2022	07/2023
ULTRAFISH	ULTRAFISH - Utilização de ultrasons na optimização da nutrição embrionária de peixes: programação nutricional do potencial de crescimento e capacidades metabólicas em juvenis.	Jorge Dias	03/2010	08/2013	190k€
SPECIAL_K	Efeitos da suplementação nutricional em vitamina K no crescimento e desenvolvimento esquelético de peixes teleósteos	Paulo Jorge Travessa Gavaia	02/2010	07/2013	195k€	195107k€

Activities

ATT: Using Zebrafish as a model in Biomedicine and study of human diseases - 3rd Edition

21st April 2017 Organization: CCMAR Location: Universidade do Algarve - Campus de Gambelas (limited places)

Group

Comparative, Adaptive and Functional Skeletal Biology

April 21, 2017

CCMAR - Universidade do Algarve - Campus de Gambelas (Faro)

Type of Event

Seminar

Fish cell lines: Current status and future challenges

Vincent Laizé will present an overview of fish cell culture, in particular cell lines capable of in vitro mineralization.

Group

Comparative, Adaptive and Functional Skeletal Biology

June 2, 2021

Online | Zoom platform

Type of Event

Scientific Gathering

IAFSB 2018: 5th Conference “Interdisciplinary Approaches in Fish Skeletal Biology”

We thank all participants for joining us at IAFSB, thus making this conference possible. IAFSB conferences would also not be possible without many hours of volunteer work of the local organising...

Group

Comparative, Adaptive and Functional Skeletal Biology

April 16, 2018 to April 19, 2018

Hotel Vila Galé Albacora, Tavira, Algarve, Portugal

Type of Event

Other Events

Interdisciplinary Approaches in Fish Skeletal Biology (IAFSB) 2018

The fifth conference “Interdisciplinary Approaches in Fish Skeletal Biology” takes place in Tavira, Portugal, April 16-19, 2018.

April 16, 2018 to April 19, 2018

Faro

Type of Event

Scientific Gathering

IAFSB 2009: 1st meeting "Interdisciplinary Approaches in Fish Skeletal Biology"

Group

Comparative, Adaptive and Functional Skeletal Biology

April 27, 2009 to April 29, 2009

Tavira, Algarve, Portugal

Type of Event

Scientific Gathering

IAFSB 2013: 3rd meeting “Interdisciplinary Approaches in Fish Skeletal Biology”

Group

Comparative, Adaptive and Functional Skeletal Biology

April 22, 2013 to April 24, 2013

Tavira, Algarve, Portugal

Type of Event

Scientific Gathering

IAFSB 2015: 4th Conference “Interdisciplinary Approaches in Fish Skeletal Biology”

Group

Comparative, Adaptive and Functional Skeletal Biology

April 27, 2015 to April 30, 2015

Tavira, Algarve, Portugal

Type of Event

Scientific Gathering

IAFSB 2011: 2nd Conference “Interdisciplinary Approaches in Fish Skeletal Biology”

Group

Comparative, Adaptive and Functional Skeletal Biology

April 26, 2011 to April 28, 2011

Tavira, Algarve, Portugal

Type of Event

Other Events

Get together CCMAR / Farewell party

Join us to a farewell party with the students from Summer School Shou (China). We're waiting for you!

July 29, 2016

Building 7 - main hall

Type of Event

Other Events

Get together CCMAR / Welcome party

Next week, join us to welcome the students from Summer School Shou (China). We're waiting for you!

July 11, 2016

Building 7 - main hall

Type of Event

Other Events

CCMAR recebe grupo de alunos da Universidade de Verão de Shou (China)

De 11 a 29 de Julho, 25 alunos da Universidade de Verão de Shou (China) vão ser acolhidos no Centro de Ciências do Mar (CCMAR) para acompanhar o trabalho dos nossos investigadores.

July 11, 2016

Bldg. 7 - Gambelas Campus

Type of Event

Seminar

GreenCoLab: Building bridges between academia and industry in algal biotechnology

GreenCoLab is a novel collaborative platform between research and industry, whose R&I agenda is based on the exploration of algae feedstocks.

May 5, 2021

Online | Zoom platform

Type of Event

Seminar

Zebrafish in vivo systems and image analysis tools to assess osteotoxicity

Marco Tarasco will talk about the use of image analysis tools in zebra fish systems in vivo to assess the toxic effect of environmental pollutants on skeletal development and bone formation.

Group

Comparative, Adaptive and Functional Skeletal Biology

March 17, 2021

Type of Event

Scientific Gathering

2020 CCMAR Symposium

The second Symposium of CCMAR will take place on 18th of December, 2020, online.

December 18, 2020

online

Type of Event

Seminar

Microalgae: a nutritional resource to improve zebrafish reproduction

Patricia Diogo will be talking about the Microalgae which have valuable nutritional value that can be used to improve zebrafish (Danio rerio) nutrition and biological performance.

July 8, 2020

Type of Event

Scientific Gathering

2019 CCMAR Symposium

The first Symposium of CCMAR will take place on 10th of May 2019, at Anfiteatro Verde da FCT, Building 9, Gambelas Campus - University of Algarve. The registrations are now open!

May 10, 2019

Anfiteatro Verde da FCT Building 9, Gambelas Campus, University of Algarve

News

Experts discuss the skeleton during this week

CCMAR organizes this week the fifth conference “Interdisciplinary Approaches in Fish Skeletal Biology (IAFSB)” in Tavira, Portugal.

In this event, the role of algae producing companies, new microalgae production strategies developed by LNEG, the concept of biorefinery of algal biomass and two round tables was presented.

Twelve young students from the high school participate in the internships that CCMAR offers in this year's edition, carried out by the CCVIVA - National Agency for Scientific Culture.

Publications

Year of Publication
2022	Tarasco M, Gavaia PJ, Bensimon-Brito A, et al. Effects of pristine or contaminated polyethylene microplastics on zebrafish development. Chemosphere. 2022;303:135198. doi:10.1016/j.chemosphere.2022.135198 Read more about Effects of pristine or contaminated polyethylene microplastics on zebrafish development Google Scholar PT DOI
2022	Rosa JT, Tarasco M, Gavaia PJ, M. Cancela L, Laizé V. Screening of Mineralogenic and Osteogenic Compounds in Zebrafish—Tools to Improve Assay Throughput and Data Accuracy. Pharmaceuticals. 2022;15(8):983. doi:10.3390/ph15080983 Read more about Screening of Mineralogenic and Osteogenic Compounds in Zebrafish—Tools to Improve Assay Throughput and Data Accuracy Google Scholar PT DOI
2022	Cardeira-da-Silva J, Bensimon-Brito A, Tarasco M, et al. Fin ray branching is defined by TRAP+ osteolytic tubules in zebrafish. Proceedings of the National Academy of Sciences. 2022;119(48). doi:10.1073/pnas.2209231119 Read more about Fin ray branching is defined by TRAP+ osteolytic tubules in zebrafish Google Scholar PT DOI
2022	Laizé V, Rosa JT, Tarasco M, M. Cancela L. Status, challenges, and perspectives of fish cell culture - Focus on cell lines capable of in vitro mineralization. In: Elsevier; 2022:381 - 404. doi:10.1016/B978-0-12-822273-7.00004-5 Read more about Status, challenges, and perspectives of fish cell culture - Focus on cell lines capable of in vitro mineralization Google Scholar PT DOI
2022	Pes K, Ortiz-Delgado JB, Sarasquete C, Laizé V, Fernández I. Short-term exposure to pharmaceuticals negatively impacts marine flatfish species: Histological, biochemical and molecular clues for an integrated ecosystem risk assessment. Environmental Toxicology and Pharmacology. 2022;90:103822. doi:10.1016/j.etap.2022.103822 Read more about Short-term exposure to pharmaceuticals negatively impacts marine flatfish species: Histological, biochemical and molecular clues for an integrated ecosystem risk assessment Google Scholar PT DOI
2022	Moreira M, Soliño L, Marques CL, et al. Cytotoxic and hemolytic activities of extracts of the fish parasite dinoflagellate Amyloodinium ocellatum. Toxins. 2022;14(7):467. doi:10.3390/toxins14070467 Read more about Cytotoxic and hemolytic activities of extracts of the fish parasite dinoflagellate Amyloodinium ocellatum Google Scholar PT DOI
2021	Cancela ML, Laizé V, Conceição N, Kempf H, Murshed M. Keutel syndrome, a review of 50 years of literature. Frontiers in Cell and Developmental Biology. 2021;9:642136. doi:10.3389/fcell.2021.642136 Read more about Keutel syndrome, a review of 50 years of literature Google Scholar PT DOI
2021	Pes K, Friese A, Cox CJ, Laizé V, Fernández I. Biochemical and molecular responses of the Mediterranean mussel (Mytilus galloprovincialis) to short-term exposure to three commonly prescribed drugs. Marine Environmental Research. 2021;168:105309. doi:10.1016/j.marenvres.2021.105309 Read more about Biochemical and molecular responses of the Mediterranean mussel (Mytilus galloprovincialis) to short-term exposure to three commonly prescribed drugs Google Scholar PT DOI
2021	Rosa JT, Laizé V, Gavaia PJ, Cancela ML. Fish models of induced osteoporosis. Frontiers in Cell and Developmental Biology. 2021;9:672424. doi:10.3389/fcell.2021.672424 Read more about Fish models of induced osteoporosis Google Scholar PT DOI
2021	Varela T, Conceição N, Laizé V, Cancela ML. Transcriptional regulation of human DUSP4 gene by cancer‐related transcription factors. Journal of Cellular Biochemistry. 2021;122(10):1556 - 1566. doi:10.1002/jcb.v122.1010.1002/jcb.30078 Read more about Transcriptional regulation of human DUSP4 gene by cancer‐related transcription factors Google Scholar PT DOI
2021	Roberto VP, Surget G, Le Lann K, et al. Antioxidant, mineralogenic and osteogenic activities of Spartina alterniflora and Salicornia fragilis extracts rich in polyphenols. Frontiers in Nutrition. 2021;8:719438. doi:10.3389/fnut.2021.71943810.3389/fnut.2021.719438.s001 Read more about Antioxidant, mineralogenic and osteogenic activities of Spartina alterniflora and Salicornia fragilis extracts rich in polyphenols Google Scholar PT DOI
2021	Tarasco M, Gavaia PJ, Bensimon-Brito A, et al. New insights into benzo[⍺]pyrene osteotoxicity in zebrafish. Ecotoxicology and Environmental Safety. 2021;226:112838. doi:10.1016/j.ecoenv.2021.112838 Read more about New insights into benzo[⍺]pyrene osteotoxicity in zebrafish Google Scholar PT DOI
2020	Vijayakumar P, Cardeira J, Laizé V, Gavaia PJ, M. Cancela L. Cells Isolated from Regenerating Caudal Fin of Sparus aurata Can Differentiate into Distinct Bone Cell Lineages. Marine Biotechnology. 2020;22(3):333 - 347. doi:10.1007/s10126-019-09937-3 Read more about Cells Isolated from Regenerating Caudal Fin of Sparus aurata Can Differentiate into Distinct Bone Cell Lineages Google Scholar PT DOI
2020	Viegas MN, Laizé V, Salgado MA, Aguiar C, Dias J. Effect of Trace Minerals and B Vitamins on the Proliferation/Cytotoxicity and Mineralization of a Gilthead Seabream Bone–Derived Cell Line. Biological Trace Element Research. 2020;196(2):629 - 638. doi:10.1007/s12011-019-01939-5 Read more about Effect of Trace Minerals and B Vitamins on the Proliferation/Cytotoxicity and Mineralization of a Gilthead Seabream Bone–Derived Cell Line Google Scholar PT DOI
2020	Varela T, Laizé V, Conceição N, et al. Expression of DUSP4 transcript variants as a potential biomarker for colorectal cancer. Biomarkers in Medicine. 2020;14(8):639 - 650. doi:10.2217/bmm-2019-0369 Read more about Expression of DUSP4 transcript variants as a potential biomarker for colorectal cancer Google Scholar PT DOI
2020	Vijayakumar P, M. Cancela L, Laizé V. Isolation, Culture, and Differentiation of Blastema Cells from the Regenerating Caudal Fin of Zebrafish. Fishes. 2020;5(1):6. doi:10.3390/fishes5010006 Read more about Isolation, Culture, and Differentiation of Blastema Cells from the Regenerating Caudal Fin of Zebrafish Google Scholar PT DOI
2020	Tarasco M, Martins G, Gavaia PJ, Bebianno MJ, M. Cancela L, Laizé V. ZEB316: A Small Stand-Alone Housing System to Study Microplastics in Small Teleosts. Zebrafish. 2020;17(1):18-26. doi:10.1089/zeb.2019.1801 Read more about ZEB316: A Small Stand-Alone Housing System to Study Microplastics in Small Teleosts Google Scholar PT DOI
2020	Tarasco M, Cordelières FP, M. Cancela L, Laizé V. ZFBONE: An ImageJ toolset for semi-automatic analysis of zebrafish bone structures. Bone. 2020;138:115480. doi:10.1016/j.bone.2020.115480 Read more about ZFBONE: An ImageJ toolset for semi-automatic analysis of zebrafish bone structures Google Scholar DOI
2020	Beato S, Marques C, Laizé V, Gavaia PJ, Fernández I. New Insights on Vitamin K Metabolism in Senegalese sole (Solea senegalensis) Based on Ontogenetic and Tissue-Specific Vitamin K Epoxide Reductase Molecular Data. International Journal of Molecular Sciences. 2020;21(10):3489. doi:10.3390/ijms21103489 Read more about New Insights on Vitamin K Metabolism in Senegalese sole (Solea senegalensis) Based on Ontogenetic and Tissue-Specific Vitamin K Epoxide Reductase Molecular Data Google Scholar PT DOI
2019	Navarro-Guillén C, Dias J, Rocha F, et al. Does a ghrelin stimulus during zebrafish embryonic stage modulate its performance on the long-term?. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 2019;228:1 - 8. doi:10.1016/j.cbpa.2018.10.019 Read more about Does a ghrelin stimulus during zebrafish embryonic stage modulate its performance on the long-term? Google Scholar DOI
2019	Tarasco M, Cardeira J, Viegas M, et al. Anti-osteogenic activity of cadmium in zebrafish. Fishes. 2019;4(1):11. doi:10.3390/fishes4010011 Read more about Anti-osteogenic activity of cadmium in zebrafish Google Scholar DOI
2019	Granadeiro L, Dirks RP, Ortiz-Delgado JB, et al. Warfarin-exposed zebrafish embryos resembles human warfarin embryopathy in a dose and developmental-time dependent manner – From molecular mechanisms to environmental concerns. Ecotoxicology and Environmental Safety. 2019;181:559 - 571. doi:10.1016/j.ecoenv.2019.06.042 Read more about Warfarin-exposed zebrafish embryos resembles human warfarin embryopathy in a dose and developmental-time dependent manner – From molecular mechanisms to environmental concerns Google Scholar PT DOI
2018	Fernández I, Gavaia P, Laizé V, M. Cancela L. Fish as a model to assess chemical toxicity in bone. Aquatic Toxicology. 2018;194:208 - 226. doi:10.1016/j.aquatox.2017.11.015 Read more about Fish as a model to assess chemical toxicity in bone Google Scholar DOI
2018	Rosa J, Cox CJ, M. Cancela L, Laizé V. Identification of a fish short-chain dehydrogenase/reductase associated with bone metabolism. Gene. 2018;645:137 - 145. doi:10.1016/j.gene.2017.12.021 Read more about Identification of a fish short-chain dehydrogenase/reductase associated with bone metabolism Google Scholar DOI
2018	Cardeira J, Laizé V, Martins G, M. Cancela L, Gavaia P. An overview on the teleost bone mechanophysiology. Journal of Applied Ichthyology. 2018. doi:10.1111/jai.13661 Read more about An overview on the teleost bone mechanophysiology Google Scholar DOI
2018	Carson MA, Nelson J, M. Cancela L, et al. Red algal extracts from Plocamium lyngbyanum and Ceramium secundatum stimulate osteogenic activities in vitro and bone growth in zebrafish larvae. Scientific Reports. 2018;8(1). doi:10.1038/s41598-018-26024-0 Read more about Red algal extracts from Plocamium lyngbyanum and Ceramium secundatum stimulate osteogenic activities in vitro and bone growth in zebrafish larvae Google Scholar DOI
2018	Laizé V, Gavaia P, Tarasco M, et al. Osteotoxicity of 3-methylcholanthrene in fish. Ecotoxicology and Environmental Safety. 2018;161:721 - 728. doi:10.1016/j.ecoenv.2018.06.035 Read more about Osteotoxicity of 3-methylcholanthrene in fish Google Scholar DOI
2018	Carson MA, Nelson J, M. Cancela L, et al. Screening for osteogenic activity in extracts from Irish marine organisms: The potential of Ceramium pallidum. Cray JJ, ed. PLOS ONE. 2018;13(11):e0207303. doi:10.1371/journal.pone.0207303.s008 Read more about Screening for osteogenic activity in extracts from Irish marine organisms: The potential of Ceramium pallidum Google Scholar DOI
2017	Tarasco M, Laizé V, Cardeira J, M. Cancela L, Gavaia P. The zebrafish operculum: A powerful system to assess osteogenic bioactivities of molecules with pharmacological and toxicological relevance. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2017;197:45 - 52. doi:10.1016/j.cbpc.2017.04.006 Read more about The zebrafish operculum: A powerful system to assess osteogenic bioactivities of molecules with pharmacological and toxicological relevance Google Scholar DOI
2017	Marques C, Roberto VP, Granadeiro L, et al. The xenobiotic sensor PXR in a marine flatfish species ( Solea senegalensis ): Gene expression patterns and its regulation under different physiological conditions. Marine Environmental Research. 2017;130:187-199. doi:10.1016/j.marenvres.2017.07.021 Read more about The xenobiotic sensor PXR in a marine flatfish species ( Solea senegalensis ): Gene expression patterns and its regulation under different physiological conditions Google Scholar DOI
2016	Cardeira J, Gavaia P, Fernández I, et al. Quantitative assessment of the regenerative and mineralogenic performances of the zebrafish caudal fin. Scientific Reports. 2016;6:39191. doi:10.1038/srep39191 Read more about Quantitative assessment of the regenerative and mineralogenic performances of the zebrafish caudal fin Google Scholar DOI
2014	Laizé V, Gavaia P, M. Cancela L. Fish: a suitable system to model human bone disorders and discover drugs with osteogenic or osteotoxic activities. Drug Discovery Today: Disease Models. 2014;13:29-37. doi:10.1016/j.ddmod.2014.08.001 Read more about Fish: a suitable system to model human bone disorders and discover drugs with osteogenic or osteotoxic activities Google Scholar DOI
2012	Viegas MN, Dias J, M. Cancela L, Laizé V. Polyunsaturated fatty acids regulate cell proliferation, extracellular matrix mineralization and gene expression in a gilthead seabream skeletal cell line. Journal of Applied Ichthyology. 2012;28(3):427-432. doi:10.1111/j.1439-0426.2012.01994.x Read more about Polyunsaturated fatty acids regulate cell proliferation, extracellular matrix mineralization and gene expression in a gilthead seabream skeletal cell line Google Scholar DOI
2010	M. Cancela L, Bargelloni L, Boudry P, et al. Genomic approaches in aquaculture and fisheries. In: J. Cock M, Tessmar-Raible K, Boyen C, Viard F, eds. Introduction To Marine Genomics. Introduction to marine genomics. Dordrecht: Springer Netherlands; 2010:213-286. doi:10.1007/978-90-481-8639-610.1007/978-90-481-8639-6_7 Read more about Genomic approaches in aquaculture and fisheries Google Scholar DOI
2010	Rosa J, Tiago DM, Dias J, M. Cancela L, Laizé V. Serum-specific stimulation of proliferation and mineralization of fish bone-derived cells. Journal of Applied Ichthyology. 2010;26(2):251 - 256. doi:10.1111/jai.2010.26.issue-210.1111/j.1439-0426.2010.01414.x Read more about Serum-specific stimulation of proliferation and mineralization of fish bone-derived cells Google Scholar DOI
2010	Rafael MS, Marques CL, Parameswaran V, M. Cancela L, Laizé V. Fish bone-derived cell lines: an alternative in vitro cell system to study bone biology. Journal of Applied Ichthyology. 2010;26(2):230-234. doi:10.1111/jai.2010.26.issue-210.1111/j.1439-0426.2010.01411.x Read more about Fish bone-derived cell lines: an alternative in vitro cell system to study bone biology Google Scholar DOI
2006	Laizé V, Viegas CSB, Price PA, M. Cancela L. Identification of an osteocalcin isoform in fish with a large acidic prodomain. Journal of Biological Chemistry. 2006;281(22):15037-15043. doi:10.1074/jbc.M600373200 Read more about Identification of an osteocalcin isoform in fish with a large acidic prodomain Google Scholar DOI
2006	Braga D, Laizé V, Tiago DM, M. Cancela L. Enhanced DNA transfer into fish bone cells using polyethylenimine. Molecular Biotechnology. 2006;34(1):51-54. doi:10.1385/MB:34:110.1385/MB:34:1:51 Read more about Enhanced DNA transfer into fish bone cells using polyethylenimine Google Scholar DOI
2005	Fonseca VG, Lago-Lestón A, Laizé V, M. Cancela L. Rapid identification of differentially expressed genes by in situ screening of bacteria. Molecular Biotechnology. 2005;30(2):163-166. doi:10.1385/MB:30:210.1385/MB:30:2:163 Read more about Rapid identification of differentially expressed genes by in situ screening of bacteria Google Scholar DOI
2005	Laizé V, Martel P, Viegas CSB, Price PA, M. Cancela L. Evolution of matrix and bone gamma-carboxyglutamic acid proteins in vertebrates. Journal of Biological Chemistry. 2005;280(29):26659-26668. doi:10.1074/jbc.M500257200 Read more about Evolution of matrix and bone gamma-carboxyglutamic acid proteins in vertebrates Google Scholar DOI